PMID- 17996337
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20071126
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 25
IP  - 50
DP  - 2007 Dec 5
TI  - Mucosal delivery of a transmission-blocking DNA vaccine encoding Giardia lamblia 
      CWP2 by Salmonella typhimurium bactofection vehicle.
PG  - 8372-83
AB  - In this study, we investigated the use of Salmonella typhimurium (STM1 strain) as 
      a bactofection vehicle to deliver a transmission-blocking DNA vaccine (TBDV) 
      plasmid to the intestinal immune system. The gene encoding the full length cyst 
      wall protein-2 (CWP2) from Giardia lamblia was subcloned into the pCDNA3 
      mammalian expression vector and stably introduced into S. typhimurium STM1. 
      Eight-week-old female BALB/c mice were orally immunized every 2 weeks, for a 
      total of three immunizations. Vaccinated and control mice were sacrificed 1 week 
      following the last injection. Administration of the DNA vaccine led to the 
      production of CWP2-specific cellular immune responses characterized by a mixed 
      Th1/Th2 response. Using ELISA, antigen-specific IgA and IgG antibodies were 
      detected in intestinal secretions. Moreover, analysis of sera demonstrated that 
      the DNA immunization also stimulated the production of CWP2-specific IgG 
      antibodies that were mainly of the IgG2a isotype. Finally, challenge infection 
      with live Giardia muris cysts revealed that mice receiving the CWP2-encoding DNA 
      vaccine were able to reduce cyst shedding by approximately 60% compared to 
      control mice. These results demonstrate, for the first time, the development of 
      parasite transmission-blocking immunity at the intestinal level following the 
      administration of a mucosal DNA vaccine delivered by S. typhimurium STM1.
FAU - Abdul-Wahid, Aws
AU  - Abdul-Wahid A
AD  - Institute of Parasitology, McGill University, 21,111 Lakeshore Road, 
      Sainte-Anne-de-Bellevue, Montreal, Quebec, Canada H9X-3V9.
FAU - Faubert, Gaetan
AU  - Faubert G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071024
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Protozoan Vaccines)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Female
MH  - Genetic Vectors
MH  - Giardia lamblia/genetics/*immunology/metabolism
MH  - Giardiasis/*prevention & control/transmission
MH  - Immunoglobulin G/blood
MH  - Intestinal Mucosa/*microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Protozoan Proteins/genetics/*immunology/metabolism
MH  - Protozoan Vaccines/administration & dosage/genetics/immunology
MH  - Salmonella typhimurium/*genetics/metabolism
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Vaccines, DNA/*administration & dosage/immunology
EDAT- 2007/11/13 09:00
MHDA- 2008/02/27 09:00
CRDT- 2007/11/13 09:00
PHST- 2007/06/27 00:00 [received]
PHST- 2007/10/01 00:00 [revised]
PHST- 2007/10/07 00:00 [accepted]
PHST- 2007/11/13 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/11/13 09:00 [entrez]
AID - S0264-410X(07)01153-X [pii]
AID - 10.1016/j.vaccine.2007.10.012 [doi]
PST - ppublish
SO  - Vaccine. 2007 Dec 5;25(50):8372-83. doi: 10.1016/j.vaccine.2007.10.012. Epub 2007 
      Oct 24.