PMID- 17997135
OWN - NLM
STAT- MEDLINE
DCOM- 20090213
LR  - 20080623
IS  - 1438-4639 (Print)
IS  - 1438-4639 (Linking)
VI  - 211
IP  - 3-4
DP  - 2008 Jul
TI  - Staphylococcus aureus of phage type 187 isolated from people occurred to be a 
      genes carrier of eneterotoxin C and toxic shock syndrome toxin-1 (TSST-1).
PG  - 273-82
AB  - The aim of this study was to examine the genotype properties of Staphylococcus 
      aureus of phage type 187 strains that constitute a separate group among the 
      strains of S. aureus. Sixteen strains were collected from the hospital patients 
      (n=12) and the healthy carriers (n=4) in 13 medical centres in Poland during 1991 
      and 2005. Biotyping, antibiotic susceptibility, phage typing, detection the genes 
      of enterotoxins and toxic shock syndrome toxin, genotyping of chromosomal DNA by 
      pulsed-field gel electrophoresis (PFGE), also amplification and restriction 
      analysis of the coagulase (coa) and the protein A genes (spa) (PCR/restriction 
      fragment length polymorphism (RFLP)) was tested. The results of this study showed 
      that all staphylococcus of phage type 187 belonged to the human biotype (A) and 
      appeared to be sensitive to all of the tested antibiotics, including methicillin 
      (MSSA). Finding out the toxin genes showed that almost all of them (93.8%) had 
      the enterotoxin C gene (sec) and TSST-1 gene (tst). The PFGE typing proved that 
      the phage type 187 strains (except for one) constitute one PFGE type. These 
      results and the identical restriction patterns in the PCR/RFLP method, also the 
      same biotype, sensitivity to antibiotics and the presence genes of the same type 
      of toxins confirmed that the phage type 187 strains constitute one clone within 
      our country. Additionally, the fact that almost all of them have the enterotoxin 
      genes and tst gene allows to consider them the strains of potentially high 
      virulence.
FAU - Piechowicz, Lidia
AU  - Piechowicz L
AD  - Department of Medical Microbiology, Medical University of Gdansk, Do Studzienki 
      38, 80-227 Gdansk, Poland. lidiap@amg.gda.pl
FAU - Garbacz, Katarzyna
AU  - Garbacz K
FAU - Galinski, Janusz
AU  - Galinski J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20071109
PL  - Germany
TA  - Int J Hyg Environ Health
JT  - International journal of hygiene and environmental health
JID - 100898843
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Coagulase)
RN  - 0 (Enterotoxins)
RN  - 0 (Staphylococcal Protein A)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 39424-54-9 (enterotoxin C, staphylococcal)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Toxins/genetics/*isolation & purification
MH  - Bacteriophage Typing
MH  - Coagulase/genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Enterotoxins/genetics/*isolation & purification
MH  - Genotype
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Poland
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Restriction Mapping
MH  - Staphylococcal Protein A/genetics
MH  - Staphylococcus aureus/*classification/drug effects/*genetics/isolation & 
      purification
MH  - Superantigens/genetics/*isolation & purification
EDAT- 2007/11/13 09:00
MHDA- 2009/02/14 09:00
CRDT- 2007/11/13 09:00
PHST- 2006/06/19 00:00 [received]
PHST- 2007/04/27 00:00 [revised]
PHST- 2007/06/18 00:00 [accepted]
PHST- 2007/11/13 09:00 [pubmed]
PHST- 2009/02/14 09:00 [medline]
PHST- 2007/11/13 09:00 [entrez]
AID - S1438-4639(07)00136-8 [pii]
AID - 10.1016/j.ijheh.2007.06.010 [doi]
PST - ppublish
SO  - Int J Hyg Environ Health. 2008 Jul;211(3-4):273-82. doi: 
      10.1016/j.ijheh.2007.06.010. Epub 2007 Nov 9.