PMID- 17997967
OWN - NLM
STAT- MEDLINE
DCOM- 20080311
LR  - 20071113
IS  - 0969-2126 (Print)
IS  - 0969-2126 (Linking)
VI  - 15
IP  - 11
DP  - 2007 Nov
TI  - Molecular interactions between a recombinant IgE antibody and the 
      beta-lactoglobulin allergen.
PG  - 1413-21
AB  - Allergies are caused by the immune reaction to commonly harmless proteins, 
      allergens. This reaction is typified by immunoglobulin E (IgE) antibodies. We 
      report the crystal structure of an IgE Fab fragment in complex with 
      beta-lactoglobulin (BLG), one of the major allergens of bovine milk. The solved 
      structure shows how two IgE/Fab molecules bind the dimeric BLG. The epitope of 
      BLG consists of six different short fragments of the polypeptide chain, which are 
      located especially in the beta strands, covering a flat area on the allergen 
      surface. All six CDR (complementary-determining region) loops of the IgE Fab 
      participate in the binding of BLG. The light chain CDR loops are responsible for 
      the binding of the flat beta sheet region of BLG. The IgE epitope is different 
      from common IgG epitopes that are normally located in the exposed loop regions of 
      antigens and observed also in the two recently determined allergen-IgG complexes.
FAU - Niemi, Merja
AU  - Niemi M
AD  - Department of Chemistry, University of Joensuu, P.O. Box 111, FIN-80101 Joensuu, 
      Finland.
FAU - Jylha, Sirpa
AU  - Jylha S
FAU - Laukkanen, Marja-Leena
AU  - Laukkanen ML
FAU - Soderlund, Hans
AU  - Soderlund H
FAU - Makinen-Kiljunen, Soili
AU  - Makinen-Kiljunen S
FAU - Kallio, Johanna M
AU  - Kallio JM
FAU - Hakulinen, Nina
AU  - Hakulinen N
FAU - Haahtela, Tari
AU  - Haahtela T
FAU - Takkinen, Kristiina
AU  - Takkinen K
FAU - Rouvinen, Juha
AU  - Rouvinen J
LA  - eng
SI  - PDB/2R56
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Structure
JT  - Structure (London, England : 1993)
JID - 101087697
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Lactoglobulins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Crystallography, X-Ray
MH  - Epitopes/chemistry/immunology
MH  - Immunoglobulin E/*chemistry/immunology
MH  - Lactoglobulins/*chemistry/*immunology
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/chemistry/immunology
EDAT- 2007/11/14 09:00
MHDA- 2008/03/12 09:00
CRDT- 2007/11/14 09:00
PHST- 2007/07/31 00:00 [received]
PHST- 2007/09/03 00:00 [revised]
PHST- 2007/09/09 00:00 [accepted]
PHST- 2007/11/14 09:00 [pubmed]
PHST- 2008/03/12 09:00 [medline]
PHST- 2007/11/14 09:00 [entrez]
AID - S0969-2126(07)00338-3 [pii]
AID - 10.1016/j.str.2007.09.012 [doi]
PST - ppublish
SO  - Structure. 2007 Nov;15(11):1413-21. doi: 10.1016/j.str.2007.09.012.