PMID- 17999180
OWN - NLM
STAT- MEDLINE
DCOM- 20080724
LR  - 20211020
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 33
IP  - 6
DP  - 2008 Jun
TI  - Chemical hypoxia facilitates alternative splicing of EAAT2 in presymptomatic 
      APP23 transgenic mice.
PG  - 1005-10
AB  - Hypoxia is one of the major common components of vascular risk factors for 
      pathogenesis of Alzheimer's disease. This study investigated the possible 
      relationship between hypoxia and alternative splicing of the excitatory amino 
      acid transporter 2 (EAAT2) in a transgenic model for Alzheimer's disease. We used 
      an APP23 mouse model prior to amyloid deposition and subjected it to chemical 
      hypoxia treatment as induced by 3-nitropropionic acid. One hour after 
      administration of 3-nitropropionic acid changes in the expression of the 
      5'-splice forms mEAAT2/5UT3, mEAAT2/5UT4, and mEAAT2/5UT5 were found in the 
      frontal cortex, hippocampus and cerebellum of the APP23 model. In untreated APP23 
      animals the expression of EAAT2 splice variants was unchanged. Our results 
      demonstrate that hypoxia facilitates alternative splicing of EAAT2 in the APP23 
      model. This may be a molecular mechanism linking vascular factors to early 
      pathophysiology of Alzheimer's disease.
FAU - Munch, Christoph
AU  - Munch C
AD  - Department of Neurology, Jewish Hospital Berlin, Heinz-Galinski-Str. 1, 13347 
      Berlin, Germany. christoph.muench@uni-ulm.de
FAU - Zhu, Bing-gen
AU  - Zhu BG
FAU - Mink, Andreas
AU  - Mink A
FAU - Seefried, Ulrich
AU  - Seefried U
FAU - Riepe, Matthias W
AU  - Riepe MW
FAU - Ludolph, Albert C
AU  - Ludolph AC
FAU - Meyer, Thomas
AU  - Meyer T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071113
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Slc1a2 protein, mouse)
SB  - IM
MH  - *Alternative Splicing
MH  - Amyloid beta-Protein Precursor/genetics/*metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - Excitatory Amino Acid Transporter 2/*genetics/metabolism
MH  - Humans
MH  - Hypoxia, Brain/chemically induced/*genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
EDAT- 2007/11/14 09:00
MHDA- 2008/07/25 09:00
CRDT- 2007/11/14 09:00
PHST- 2007/08/14 00:00 [received]
PHST- 2007/10/30 00:00 [accepted]
PHST- 2007/11/14 09:00 [pubmed]
PHST- 2008/07/25 09:00 [medline]
PHST- 2007/11/14 09:00 [entrez]
AID - 10.1007/s11064-007-9540-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2008 Jun;33(6):1005-10. doi: 10.1007/s11064-007-9540-5. Epub 2007 
      Nov 13.