PMID- 18000389
OWN - NLM
STAT- MEDLINE
DCOM- 20071203
LR  - 20071116
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 118
IP  - 2-4
DP  - 2007
TI  - Molecular cytogenetics of IGH rearrangements in non-Hodgkin B-cell lymphoma.
PG  - 345-52
AB  - Rearrangements involving the IGH gene have been identified in about 50% of 
      non-Hodgkin B-cell lymphomas (NHLs) and correlated to clinically relevant 
      subgroups. However, the detection rate largely varied with the technique used. We 
      analyzed the incidence of IGH rearrangements using several fluorescence in situ 
      hybridization (FISH) techniques on metaphases obtained from 96 patients with 
      nodal NHL. An IGH rearrangement was identified in 71 cases (74%). A 
      t(14;18)(q32;q21) was found in 37 of the 42 follicular lymphomas (88.1%) studied 
      and a t(11;14)(q13;q32) in 12 of the 14 mantle cell lymphomas (85.7%). IGH 
      rearrangements were identified in 21 of the 40 diffuse large B-cell lymphomas 
      (52.5%), including seven t(14;18)(q32;q21) and four t(3;14)(q27;q32). 
      Conventional cytogenetics was uninformative in several cases. However, the 
      complemented analysis using 24-color FISH, chromosomal whole paints, telomeric 
      probes and locus specific identifiers enabled us to characterize complex and/or 
      masked IGH translocations in follicular lymphomas and mantle cell lymphomas and 
      to identify all the chromosomal partners involved in IGH rearrangements in 
      diffuse large B-cell lymphomas. This study shows the interest of using metaphase 
      FISH in addition to conventional cytogenetics. Following banding techniques, FISH 
      with the IGH dual color probe can be the first approach in NHL, after which 
      chromosome painting and 24-color FISH can be used to identify the chromosomal 
      partners involved in IGH rearrangements. The identification of these genes is of 
      utmost importance for a better understanding of the molecular mechanisms involved 
      in the genesis of lymphoma.
CI  - Copyright (c) 2007 S. Karger AG, Basel.
FAU - Bernicot, I
AU  - Bernicot I
AD  - Laboratoire d'Histologie, Embryologie et Cytogenetique, Faculte de Medecine et 
      des Sciences de la Sante, Universite de Bretagne Occidentale, Brest, France.
FAU - Douet-Guilbert, N
AU  - Douet-Guilbert N
FAU - Le Bris, M-J
AU  - Le Bris MJ
FAU - Herry, A
AU  - Herry A
FAU - Morel, F
AU  - Morel F
FAU - De Braekeleer, M
AU  - De Braekeleer M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Metaphase
MH  - *Translocation, Genetic
EDAT- 2007/11/15 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/11/15 09:00
PHST- 2006/09/27 00:00 [received]
PHST- 2006/11/30 00:00 [accepted]
PHST- 2007/11/15 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/11/15 09:00 [entrez]
AID - 000108319 [pii]
AID - 10.1159/000108319 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2007;118(2-4):345-52. doi: 10.1159/000108319.