PMID- 18005264
OWN - NLM
STAT- MEDLINE
DCOM- 20080219
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 151
IP  - 1
DP  - 2008 Jan
TI  - Increased weight loss with reduced viral replication in interleukin-10 knock-out 
      mice infected with murine cytomegalovirus.
PG  - 155-64
AB  - The anti-inflammatory cytokine interleukin (IL)-10 plays an important role in the 
      regulation of host-immune responses. Here we studied the role IL-10 plays in host 
      responses to cytomegalovirus (CMV) infection. We demonstrate that manifestations 
      of murine CMV (MCMV) disease are more severe in IL-10 knock-out mice, despite 
      significantly reduced levels of viral replication. Cytokine analysis of serum 
      revealed increased levels of interferon (IFN)-gamma, monocyte chemotactic protein 
      1 (MCP-1) and IL-6, all of which are potent stimulators of inflammatory 
      responses. Depletion of IFN-gamma by monoclonal antibodies in IL-10 knock-out 
      mice failed to improve the physical condition of the mice, while increasing viral 
      replication. In contrast, serum levels of IL-6 in the knock-out animals were 
      unaffected by IFN-gamma depletion and remained significantly elevated early in 
      the course of infection. These data suggest that increased weight loss observed 
      in IL-10 knock-out mice may be attributed to the uncontrolled production of 
      proinflammatory cytokines, including IL-6.
FAU - Oakley, O R
AU  - Oakley OR
AD  - Department of Clinical Sciences, University of Kentucky, Lexington, KY 
      40536-0084, USA. oroakl1@email.uky.edu
FAU - Garvy, B A
AU  - Garvy BA
FAU - Humphreys, S
AU  - Humphreys S
FAU - Qureshi, M H
AU  - Qureshi MH
FAU - Pomeroy, C
AU  - Pomeroy C
LA  - eng
GR  - HL-62053/HL/NHLBI NIH HHS/United States
GR  - HL-64524/HL/NHLBI NIH HHS/United States
GR  - R15 HL084654-01A1/HL/NHLBI NIH HHS/United States
GR  - R15 HL084654/HL/NHLBI NIH HHS/United States
GR  - R01 HL062053/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071114
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Chemokine CCL2/analysis
MH  - Female
MH  - Flow Cytometry
MH  - Herpesviridae Infections/*immunology/virology
MH  - Interferon-gamma/analysis
MH  - Interleukin-10/genetics/*physiology
MH  - Interleukin-6/analysis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Muromegalovirus/*physiology
MH  - RNA, Messenger/analysis
MH  - Up-Regulation
MH  - Virus Replication
MH  - *Weight Loss
PMC - PMC2276919
EDAT- 2007/11/17 09:00
MHDA- 2008/02/20 09:00
PMCR- 2009/01/01
CRDT- 2007/11/17 09:00
PHST- 2007/11/17 09:00 [pubmed]
PHST- 2008/02/20 09:00 [medline]
PHST- 2007/11/17 09:00 [entrez]
PHST- 2009/01/01 00:00 [pmc-release]
AID - CEI3533 [pii]
AID - 10.1111/j.1365-2249.2007.03533.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2008 Jan;151(1):155-64. doi: 10.1111/j.1365-2249.2007.03533.x. 
      Epub 2007 Nov 14.