PMID- 18005361
OWN - NLM
STAT- MEDLINE
DCOM- 20080212
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 151
IP  - 2
DP  - 2008 Feb
TI  - Adhesion molecules involved in hepoxilin A3-mediated neutrophil transepithelial 
      migration.
PG  - 297-305
AB  - A common feature underlying active states of inflammation is the migration of 
      neutrophils (PMNs) from the circulation and across a number of tissue barriers in 
      response to chemoattractant stimuli. Although our group has recently established 
      a discreet role for the PMN chemoattractant, hepoxilin A3 (HXA3) in the process 
      of PMN recruitment, very little is known regarding the interaction of HXA3 with 
      PMNs. To characterize further the event of HXA3-induced PMN transepithelial 
      migration, we sought to determine the adhesion molecules required for migration 
      across different epithelial surfaces (T84 intestinal and A549 airway cells) 
      relative to two well-studied PMN chemoattractants, 
      formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B4 (LTB4). Our 
      findings reveal that the adhesion interaction profile of PMN transepithelial 
      migration in response to HXA3 differs from the adhesion interaction profile 
      exhibited by the structurally related eicosanoid LTB4. Furthermore, unique to PMN 
      transepithelial migration induced by gradients of HXA3 was the critical 
      dependency of all four major surface adhesion molecules examined (i.e. CD18, 
      CD47, CD44 and CD55). Our results suggest that the particular chemoattractant 
      gradient imposed, as well as the type of epithelial cell monolayer, each plays a 
      role in determining the adhesion molecules involved in transepithelial migration. 
      Given the complexities of these interactions, our findings are important to 
      consider with respect to adhesion molecules that may be targeted for potential 
      drug development.
FAU - Hurley, B P
AU  - Hurley BP
AD  - Mucosal Immunology Laboratory, Department of Pediatric Gastroenterology and 
      Nutrition, Massachusetts General Hospital, Boston, MA 02129, USA.
FAU - Sin, A
AU  - Sin A
FAU - McCormick, B A
AU  - McCormick BA
LA  - eng
GR  - P30 DK040561/DK/NIDDK NIH HHS/United States
GR  - DK 33506/DK/NIDDK NIH HHS/United States
GR  - P30 DK040561-12/DK/NIDDK NIH HHS/United States
GR  - R01 DK056754/DK/NIDDK NIH HHS/United States
GR  - DK 56754/DK/NIDDK NIH HHS/United States
GR  - P01 DK033506/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071115
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemotactic Factors)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 85589-24-8 (8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 8,11,14-Eicosatrienoic Acid/*analogs & derivatives/pharmacology
MH  - Cell Adhesion Molecules/*physiology
MH  - Chemotactic Factors/pharmacology
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/cytology
MH  - Humans
MH  - Leukotriene B4/pharmacology
MH  - Neutrophils/*drug effects/physiology
MH  - Tumor Cells, Cultured
PMC - PMC2276941
EDAT- 2007/11/17 09:00
MHDA- 2008/02/13 09:00
PMCR- 2009/02/01
CRDT- 2007/11/17 09:00
PHST- 2007/11/17 09:00 [pubmed]
PHST- 2008/02/13 09:00 [medline]
PHST- 2007/11/17 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - CEI3551 [pii]
AID - 10.1111/j.1365-2249.2007.03551.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2008 Feb;151(2):297-305. doi: 10.1111/j.1365-2249.2007.03551.x. 
      Epub 2007 Nov 15.