PMID- 18006657
OWN - NLM
STAT- MEDLINE
DCOM- 20080115
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 104
IP  - 48
DP  - 2007 Nov 27
TI  - Vaginal epithelial dendritic cells renew from bone marrow precursors.
PG  - 19061-6
AB  - Dendritic cells (DCs) represent key professional antigen-presenting cells capable 
      of initiating primary immune responses. A specialized subset of DCs, the 
      Langerhans cells (LCs), are located in the stratified squamous epithelial layer 
      of the skin and within the mucosal epithelial lining of the vaginal and oral 
      cavities. The vaginal mucosa undergoes cyclic changes under the control of sex 
      hormones, and the renewal characteristics of the vaginal epithelial DCs (VEDCs) 
      remain unknown. Here, we examined the origin of VEDCs. In contrast to the skin 
      epidermal LCs, the DCs in the epithelium of the vagina were found to be 
      repopulated mainly by nonmonocyte bone-marrow-derived precursors, with a 
      half-life of 13 days under steady-state conditions. Upon infection with HSV-2, 
      the Gr-1(hi) monocytes were found to give rise to VEDCs. Furthermore, flow 
      cytometric analysis of the VEDCs revealed the presence of at least three distinct 
      populations, namely, CD11b(+)F4/80(hi), CD11b(+)F4/80(int), and CD11b(-)F4/80(-). 
      Importantly, these VEDC populations expressed CD207 at low levels and had a 
      constitutively more activated phenotype compared with the skin LCs. Collectively, 
      our results revealed mucosa-specific features of the VEDCs with respect to their 
      phenotype, activation status, and homeostatic renewal potential.
FAU - Iijima, Norifumi
AU  - Iijima N
AD  - Department of Immunobiology, Yale University School of Medicine, New Haven, CT 
      06520, USA.
FAU - Linehan, Melissa M
AU  - Linehan MM
FAU - Saeland, Sem
AU  - Saeland S
FAU - Iwasaki, Akiko
AU  - Iwasaki A
LA  - eng
GR  - AI064705/AI/NIAID NIH HHS/United States
GR  - AI054359/AI/NIAID NIH HHS/United States
GR  - R01 AI064705/AI/NIAID NIH HHS/United States
GR  - R01 AI054359/AI/NIAID NIH HHS/United States
GR  - R56 AI062428/AI/NIAID NIH HHS/United States
GR  - AI062428/AI/NIAID NIH HHS/United States
GR  - R01 AI062428/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071115
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Surface)
RN  - 0 (Cd207 protein, mouse)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Animals, Congenic
MH  - Antigens, CD/analysis
MH  - Antigens, Surface/biosynthesis
MH  - Bone Marrow Cells/*cytology
MH  - *Cell Lineage
MH  - Diestrus
MH  - Epidermal Cells
MH  - Epithelial Cells/*cytology
MH  - Female
MH  - Herpes Genitalis/pathology
MH  - Lectins, C-Type/biosynthesis
MH  - Lymph Nodes/cytology
MH  - Mannose-Binding Lectins/biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Mucous Membrane/cytology
MH  - Multipotent Stem Cells/*cytology
MH  - Organ Specificity
MH  - Radiation Chimera
MH  - Vagina/*cytology
PMC - PMC2141908
COIS- The authors declare no conflict of interest.
EDAT- 2007/11/17 09:00
MHDA- 2008/01/16 09:00
PMCR- 2008/05/27
CRDT- 2007/11/17 09:00
PHST- 2007/11/17 09:00 [pubmed]
PHST- 2008/01/16 09:00 [medline]
PHST- 2007/11/17 09:00 [entrez]
PHST- 2008/05/27 00:00 [pmc-release]
AID - 0707179104 [pii]
AID - 8343 [pii]
AID - 10.1073/pnas.0707179104 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19061-6. doi: 
      10.1073/pnas.0707179104. Epub 2007 Nov 15.