PMID- 1801722
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20061115
IS  - 0232-766X (Print)
IS  - 0232-766X (Linking)
VI  - 50
IP  - 4-6
DP  - 1991
TI  - Detection of cathepsin B in tumor cytosol and urine of breast cancer patients.
PG  - 555-60
AB  - In order to evaluate the role of cathepsin B (CB) as a proteinase involved in 
      mammary tumor progression and its potential role as a tumor marker, we have 
      measured the CB activity in cytosols of breast cancer tumor tissue using 
      Z-Arg-Arg-AMC as the substrate and found a 23fold increase when compared to 
      distantly located breast tissue from the same patient. In addition, urine of 
      breast cancer patients under adjuvant chemotherapy was screened for CB 
      immunoreactivity with a sandwich type enzyme immunoassay which revealed 
      significant interindividual differences in concentrations with some urines 
      containing immunoreactivity comparable to healthy controls. The urines were also 
      investigated for CB activity but no differences between patients and controls 
      were observed. However, urine contains thiol activatable proteinases causing 
      substrate hydrolysis, which is to a varying extent inhibited by E-64 or 
      Z-Phe-Phe-CHN2.
FAU - Dengler, R
AU  - Dengler R
AD  - Medizinische Klinik Innenstadt, Universitat Munchen, FRG.
FAU - Lah, T
AU  - Lah T
FAU - Gabrijelcic, D
AU  - Gabrijelcic D
FAU - Turk, V
AU  - Turk V
FAU - Fritz, H
AU  - Fritz H
FAU - Emmerich, B
AU  - Emmerich B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biomed Biochim Acta
JT  - Biomedica biochimica acta
JID - 8304435
RN  - 0 (Coumarins)
RN  - 0 (Dipeptides)
RN  - 0 (Fluorescent Dyes)
RN  - 88937-61-5 (benzyloxycarbonylarginyl-arginine 4-methylcoumarin-7-ylamide)
RN  - EC 3.4.22.1 (Cathepsin B)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/*enzymology/urine
MH  - Cathepsin B/antagonists & inhibitors/*metabolism/urine
MH  - Coumarins
MH  - Cytosol/enzymology
MH  - Dipeptides
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - Middle Aged
MH  - Substrate Specificity
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Biomed Biochim Acta. 1991;50(4-6):555-60.