PMID- 18024081
OWN - NLM
STAT- MEDLINE
DCOM- 20080509
LR  - 20131121
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 25
IP  - 1
DP  - 2008 Jan
TI  - Steroid secretion following exposure of ovarian follicular cells to three 
      different natural mixtures of persistent organic pollutants (POPs).
PG  - 58-66
AB  - This study investigated in vitro endocrine disrupting effects of three mixtures 
      of POPs: 'Marine mix' extracted from Atlantic cod liver, and two mixtures 
      extracted from burbot liver, 'Mjosa mix' and 'Losna mix'. The POP mixtures were 
      chemically characterized. Co-culture of theca and granulosa cells, were exposed 
      for 48h with different doses of 'Marine mix', 'Mjosa mix' or 'Losna mix'. As an 
      end point cell viability was determinated by LDH test, steroid analysis by EIA 
      and caspase-3 by colorimetric substrate. Chemical characterization of the 
      mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and 
      PCBs. In the 'Mjosa mix', the dominant pollutants were BDEs and HBCD. The 
      concentrations of POPs measured in the 'Losna mix' were considerably lower. All 
      mixtures used in the present study had a stimulatory effect on testosterone and 
      estradiol secretion with 'Marine mix'>'Mjosa mix'>'Losna mix'. These results show 
      that even a mixture containing background concentrations of POPs significantly 
      affected steroid secretion. A higher steroidogenic response in low dose ranges, 
      compared with high dose ranges indicated xenobiotic-conditioning hormesis. This 
      could complicate predictions of effects in risk assessments.
FAU - Gregoraszczuk, Ewa L
AU  - Gregoraszczuk EL
AD  - Department of Physiology and Toxicology of Reproduction, Institute of Zoology, 
      Jagiellonian University, Ingardena 6, 30-060 Krakow, Poland. 
      greg@zuk.iz.uj.edu.pl
FAU - Milczarek, Katarzyna
AU  - Milczarek K
FAU - Wojtowicz, Anna K
AU  - Wojtowicz AK
FAU - Berg, Vidar
AU  - Berg V
FAU - Skaare, Janneche U
AU  - Skaare JU
FAU - Ropstad, Erik
AU  - Ropstad E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071009
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Fish Proteins)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Water Pollutants, Chemical)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 8001-69-2 (Cod Liver Oil)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cod Liver Oil/chemistry/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Endocrine Disruptors/*chemistry/isolation & purification
MH  - Estradiol/metabolism
MH  - Female
MH  - Fish Proteins/metabolism
MH  - Gadiformes
MH  - Gadus morhua
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Granulosa Cells/*drug effects/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Norway
MH  - Progesterone/metabolism
MH  - Risk Assessment
MH  - Swine
MH  - Theca Cells/*drug effects/metabolism
MH  - Time Factors
MH  - Water Pollutants, Chemical/isolation & purification/*toxicity
EDAT- 2007/11/21 09:00
MHDA- 2008/05/10 09:00
CRDT- 2007/11/21 09:00
PHST- 2007/06/05 00:00 [received]
PHST- 2007/09/20 00:00 [revised]
PHST- 2007/10/02 00:00 [accepted]
PHST- 2007/11/21 09:00 [pubmed]
PHST- 2008/05/10 09:00 [medline]
PHST- 2007/11/21 09:00 [entrez]
AID - S0890-6238(07)00281-X [pii]
AID - 10.1016/j.reprotox.2007.10.001 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2008 Jan;25(1):58-66. doi: 10.1016/j.reprotox.2007.10.001. Epub 
      2007 Oct 9.