PMID- 18025190
OWN - NLM
STAT- MEDLINE
DCOM- 20080205
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 179
IP  - 11
DP  - 2007 Dec 1
TI  - Functional role of P-selectin glycoprotein ligand 1/P-selectin interaction in the 
      generation of tolerogenic dendritic cells.
PG  - 7457-65
AB  - Dendritic cells (DCs) have a key role in both the generation of the immune 
      response and the induction of tolerance to self-Ags. In this work, the possible 
      role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of 
      human DCs was explored. We found that the engagement of PSGL-1 by P-selectin on 
      DCs induced the expression of c-Fos, IDO, IL-10, and TGF-beta genes. Remarkably, 
      stimulation of DCs through PSGL-1 with P-selectin enhanced their capability to 
      generate CD4(+)CD25(+)Foxp3(+) regulatory T cells, which expressed high levels of 
      TGF-beta1 mRNA, synthesized IL-10, and suppressed the proliferation of autologous 
      CD4(+)CD25(-) T cells. Accordingly, we found that DCs from PSGL-1(-/-) mice 
      expressed higher levels of MHC class II molecules, and exhibited an enhanced 
      immunogenicity compared with wild-type mice. In addition, the percentage of 
      CD4(+)CD25(+)Foxp3(+) regulatory T cells in the thymus of PSGL-1-deficient 
      animals was significantly reduced. Our data reveal an unexpected role of PSGL-1 
      on the tolerogenic function of DCs, and the regulation of the immune response.
FAU - Urzainqui, Ana
AU  - Urzainqui A
AD  - Servicio de Inmunologia, Hospital Universitario de La Princesa, Universidad 
      Autonoma de Madrid, Madrid, Spain.
FAU - Martinez del Hoyo, Gloria
AU  - Martinez del Hoyo G
FAU - Lamana, Amalia
AU  - Lamana A
FAU - de la Fuente, Hortensia
AU  - de la Fuente H
FAU - Barreiro, Olga
AU  - Barreiro O
FAU - Olazabal, Isabel M
AU  - Olazabal IM
FAU - Martin, Pilar
AU  - Martin P
FAU - Wild, Martin K
AU  - Wild MK
FAU - Vestweber, Dietmar
AU  - Vestweber D
FAU - Gonzalez-Amaro, Roberto
AU  - Gonzalez-Amaro R
FAU - Sanchez-Madrid, Francisco
AU  - Sanchez-Madrid F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (P-Selectin)
RN  - 0 (P-selectin ligand protein)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/immunology
MH  - Female
MH  - Forkhead Transcription Factors/immunology
MH  - Gene Expression Regulation/genetics
MH  - Immune Tolerance/*immunology
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics
MH  - Interleukin-10/genetics
MH  - Male
MH  - Membrane Glycoproteins/biosynthesis/genetics/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - P-Selectin/*metabolism/pharmacology
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - RNA, Messenger/genetics
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Transforming Growth Factor beta/genetics
MH  - Transforming Growth Factor beta1/genetics
EDAT- 2007/11/21 09:00
MHDA- 2008/02/06 09:00
CRDT- 2007/11/21 09:00
PHST- 2007/11/21 09:00 [pubmed]
PHST- 2008/02/06 09:00 [medline]
PHST- 2007/11/21 09:00 [entrez]
AID - 179/11/7457 [pii]
AID - 10.4049/jimmunol.179.11.7457 [doi]
PST - ppublish
SO  - J Immunol. 2007 Dec 1;179(11):7457-65. doi: 10.4049/jimmunol.179.11.7457.