PMID- 18026219
OWN - NLM
STAT- MEDLINE
DCOM- 20080229
LR  - 20161124
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 53
IP  - 11
DP  - 2007 Nov
TI  - Production of active pediocin PA-1 in Escherichia coli using a thioredoxin gene 
      fusion expression approach: cloning, expression, purification, and 
      characterization.
PG  - 1246-58
AB  - Antimicrobial peptides possess cationic and amphipathic properties that allow for 
      interactions with the membrane of living cells. Bacteriocins from lactic acid 
      bacteria, in particular, are currently being studied for their potential use as 
      food preservatives and for applications in health care. However, bacteriocin 
      exploitation is often limited owing to low production yields. Gene cloning and 
      heterologous protein or peptide production is one way to possibly achieve 
      overexpression of bacteriocins to support biochemical studies. In this work, 
      production of recombinant active pediocin PA-1 (PedA) was accomplished in 
      Escherichia coli using a thioredoxin (trx) gene fusion (trx-pedA) expression 
      approach. Trx-PedA itself did not show any biological activity, but upon cleavage 
      by an enterokinase, biologically active pediocin PA-1 was obtained. Recombinant 
      pediocin PA-1 characteristics (molecular mass, biological activity, 
      physicochemical properties) were very similar to those of native pediocin PA-1. 
      In addition, a 4- to 5-fold increase in production yield was obtained, by 
      comparison with the PA-1 produced naturally by Pediococcus acidilactici PAC 1.0. 
      The new production method, although not optimized, offers great potential for 
      supporting further investigations on pediocin PA-1 and as a first-generation 
      process for the production of pediocin PA-1 for high-value applications.
FAU - Beaulieu, Lucie
AU  - Beaulieu L
AD  - Biotechnology Research Institute, National Research Council, 6100 Royalmount 
      Avenue, Montreal, QC 4P 2R2, Canada.
FAU - Tolkatchev, Dmitri
AU  - Tolkatchev D
FAU - Jette, Jean-Francois
AU  - Jette JF
FAU - Groleau, Denis
AU  - Groleau D
FAU - Subirade, Muriel
AU  - Subirade M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 0 (Bacteriocins)
RN  - 0 (Pediocins)
RN  - 0 (Recombinant Proteins)
RN  - 111745-56-3 (pediocin PA-1)
RN  - 52500-60-4 (Thioredoxins)
SB  - IM
MH  - Bacteriocins/*biosynthesis/chemistry/genetics
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - *Gene Expression Regulation, Bacterial
MH  - *Gene Fusion
MH  - Pediocins
MH  - Pediococcus/genetics
MH  - Recombinant Proteins/metabolism
MH  - Thioredoxins/*genetics/metabolism
EDAT- 2007/11/21 09:00
MHDA- 2008/03/01 09:00
CRDT- 2007/11/21 09:00
PHST- 2007/11/21 09:00 [pubmed]
PHST- 2008/03/01 09:00 [medline]
PHST- 2007/11/21 09:00 [entrez]
AID - w07-089 [pii]
AID - 10.1139/w07-089 [doi]
PST - ppublish
SO  - Can J Microbiol. 2007 Nov;53(11):1246-58. doi: 10.1139/w07-089.