PMID- 18027361
OWN - NLM
STAT- MEDLINE
DCOM- 20080305
LR  - 20071217
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 30
IP  - 18
DP  - 2007 Dec
TI  - Pesticide analysis in rose wines by micellar electrokinetic chromatography.
PG  - 3240-6
AB  - In this work, the determination of 11 pesticides (pirimicarb, metalaxyl, 
      pyrimethanil, procymidone, nuarimol, azoxystrobin, tebufenozide, fenarimol, 
      benalaxyl, penconazole, and tetradifon) in rose wines by micellar EKC (MEKC) 
      using reversed electrode polarity stacking mode (REPSM) as online 
      preconcentration strategy is described. The MEKC buffer consisted of 100 mM 
      sodium tetraborate and 30 mM SDS at pH 8.5 with 6% v/v 1-propanol. A solid-phase 
      microextraction (SPME) procedure using PDMS/divinylbenzene (PDMS/DVB) fibers was 
      applied to extract the selected pesticides from the rose wine samples. The 
      comparison between the calibration curves obtained from hydroalcoholic solutions 
      (12% v/v ethanol) and from rose wines (matrix matched calibration) showed the 
      existence of a strong matrix effect. Furthermore, a comparison with calibration 
      curves obtained with white wine samples also showed significant differences for 
      most of the analyzed pesticides. As a result, a matrix matched calibration was 
      developed. Quantitative extraction from spiked wine samples was carried out in 
      triplicate at two levels of concentration (range 0.18-6.00 mg/L). LODs between 
      0.040 and 0.929 mg/L were achieved, which are below the maximum residue limits 
      (MRLs) established for wine grapes (except for pirimicarb) by the EU and Spain 
      legislation as well as by the Codex Alimentarius. The established method - which 
      is solvent free, cost effective, and fast - was also applied to the analysis of 
      several homemade rose wine samples and a commercial one. Two of the selected 
      pesticides were found in some of the analyzed samples.
FAU - Ravelo-Perez, Lidia M
AU  - Ravelo-Perez LM
AD  - Department of Analytical Chemistry, Nutrition and Food Science, University of La 
      Laguna, Tenerife, Canary Islands, Spain.
FAU - Hernandez-Borges, Javier
AU  - Hernandez-Borges J
FAU - Borges-Miquel, Teresa M
AU  - Borges-Miquel TM
FAU - Rodriguez-Delgado, Miguel Angel
AU  - Rodriguez-Delgado MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Pesticides)
SB  - IM
MH  - Calibration
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Pesticides/*analysis
MH  - Reproducibility of Results
MH  - Rosa/*chemistry
MH  - Wine/*analysis
EDAT- 2007/11/21 09:00
MHDA- 2008/03/06 09:00
CRDT- 2007/11/21 09:00
PHST- 2007/11/21 09:00 [pubmed]
PHST- 2008/03/06 09:00 [medline]
PHST- 2007/11/21 09:00 [entrez]
AID - 10.1002/jssc.200700288 [doi]
PST - ppublish
SO  - J Sep Sci. 2007 Dec;30(18):3240-6. doi: 10.1002/jssc.200700288.