PMID- 18027547
OWN - NLM
STAT- MEDLINE
DCOM- 20080123
LR  - 20191110
IS  - 0015-6426 (Print)
IS  - 0015-6426 (Linking)
VI  - 48
IP  - 5
DP  - 2007 Oct
TI  - Determination method for ractopamine in swine and cattle tissues using LC/MS.
PG  - 144-7
AB  - Simple and reliable methods using LC/MS have been developed for the determination 
      of the beta-agonist ractopamine in swine and cattle tissues. Ractopamine was 
      extracted with ethyl acetate from muscle and liver, and the ethyl acetate layer 
      was evaporated to dryness. The residue was purified by partition with 
      acetonitrile/n-hexane. In the case of fat, ractopamine was extracted and purified 
      by partition with acetonitrile/n-hexane. The resulting acetonitrile solutions 
      were evaporated to dryness. The residue was dissolved in methanol, and subjected 
      to LC/MS. The LC separation was performed on a Wakosil-II 3C18HG column (150 x 3 
      mm i.d.) in isocratic mode with 0.05% trifluoroacetic acid-acetonitrile (80:20) 
      as a mobile phase at a flow rate of 0.4 mL/min. The MS detection was performed in 
      the selected ion recording (SIR) mode, with detection of the M + H+ ion of 
      ractopamine (m/z 302) produced by electrospray ionization (ESI). The mean 
      recoveries of the drug from swine muscle (0.01 microg fortified), fat (0.01 
      microg fortified) and liver (0.04 microg/g fortified) were 99.7%, 99.5% and 
      100.8%, and those from cattle samples were 108.3%, 97.0% and 109.4%, 
      respectively. The relative standard deviations (RSDs) ranged from 0.1% to 9.5%. 
      The limit of quantification (LOQ) of the drug was 1 ng/g.
FAU - Sakai, Takatoshi
AU  - Sakai T
AD  - National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 
      158-8501, Japan.
FAU - Hitomi, Tomomi
AU  - Hitomi T
FAU - Sugaya, Kyoko
AU  - Sugaya K
FAU - Kai, Shigemi
AU  - Kai S
FAU - Murayama, Mitsunori
AU  - Murayama M
FAU - Maitani, Tamio
AU  - Maitani T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Shokuhin Eiseigaku Zasshi
JT  - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
JID - 0142214
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Phenethylamines)
RN  - 57370OZ3P1 (ractopamine)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis
MH  - Animals
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Mass Spectrometry
MH  - Meat/*analysis
MH  - Phenethylamines/*analysis
MH  - Swine
EDAT- 2007/11/22 09:00
MHDA- 2008/01/24 09:00
CRDT- 2007/11/22 09:00
PHST- 2007/11/22 09:00 [pubmed]
PHST- 2008/01/24 09:00 [medline]
PHST- 2007/11/22 09:00 [entrez]
AID - 10.3358/shokueishi.48.144 [doi]
PST - ppublish
SO  - Shokuhin Eiseigaku Zasshi. 2007 Oct;48(5):144-7. doi: 10.3358/shokueishi.48.144.