PMID- 18028053
OWN - NLM
STAT- MEDLINE
DCOM- 20080219
LR  - 20191210
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 115
IP  - 6
DP  - 2007 Dec
TI  - In situ identification of streptococci and other bacteria in initial dental 
      biofilm by confocal laser scanning microscopy and fluorescence in situ 
      hybridization.
PG  - 459-67
AB  - Confocal laser scanning microscopy (CLSM) has been employed as a method for 
      studying intact natural biofilm. When combined with fluorescence in situ 
      hybridization (FISH) it is possible to analyze spatial relationships and changes 
      of specific members of microbial populations over time. The aim of this study was 
      to perform a systematic description of the pattern of initial dental biofilm 
      formation by applying 16S rRNA-targeted oligonucleotide probes to the 
      identification of streptococci and other bacteria, and to evaluate the usefulness 
      of the combination of CLSM and FISH for structural studies of bacterial 
      populations in dental biofilm. Biofilms were collected on standardized glass 
      slabs mounted in intra-oral appliances and worn by 10 individuals for 6, 12, 24 
      or 48 h. After intra-oral exposure the biofilms were labelled with probes against 
      either streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. The 
      current approach of using FISH techniques enabled differentiation of streptococci 
      from other bacteria and determination of their spatio-temporal organization. The 
      presence of chimney-like multilayered microcolonies with different microbial 
      compositions demonstrated by this methodology provided information supplementary 
      to our previous knowledge obtained by classical electron microscopic methods and 
      increased our understanding of the structure of developing biofilms.
FAU - Dige, Irene
AU  - Dige I
AD  - Department of Dental Pathology, Operative Dentistry and Endodontics, School of 
      Dentistry, University of Aarhus, Arhus, Denmark. idige@odont.au.dk
FAU - Nilsson, Holger
AU  - Nilsson H
FAU - Kilian, Mogens
AU  - Kilian M
FAU - Nyvad, Bente
AU  - Nyvad B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (DNA Probes)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - *Biofilms
MH  - DNA Probes/genetics
MH  - Dental Plaque/*microbiology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Microscopy, Confocal/methods
MH  - Oligonucleotide Probes
MH  - RNA, Ribosomal, 16S/*analysis
MH  - Streptococcus/genetics/*isolation & purification
MH  - Time Factors
EDAT- 2007/11/22 09:00
MHDA- 2008/02/20 09:00
CRDT- 2007/11/22 09:00
PHST- 2007/11/22 09:00 [pubmed]
PHST- 2008/02/20 09:00 [medline]
PHST- 2007/11/22 09:00 [entrez]
AID - EOS494 [pii]
AID - 10.1111/j.1600-0722.2007.00494.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2007 Dec;115(6):459-67. doi: 10.1111/j.1600-0722.2007.00494.x.