PMID- 18028701
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20071121
IS  - 0003-7028 (Print)
IS  - 0003-7028 (Linking)
VI  - 61
IP  - 11
DP  - 2007 Nov
TI  - Core protein-nucleic acid interactions in hepatitis C virus as revealed by Raman 
      and circular dichroism spectroscopy.
PG  - 1219-24
AB  - Molecular interactions required for hepatitis C virus (HCV) assembly are not well 
      known and are poorly understood. The 5' untranslated region (5'UTR) of the RNA 
      genome is highly conserved and has extensive secondary structure, and the highly 
      basic core protein is rich in arginine residues. Using Raman and circular 
      dichroism (CD) spectroscopies, specific interactions have been demonstrated here 
      between the 5'UTR sequence and the core protein that may be important for the 
      specific encapsidation of the viral genome during HCV replication. These 
      interactions can be described as follows: (1) hydrogen bonding of arginine with 
      unpaired guanine and/or with wobble GU base pairs, and arginine-phosphate 
      electrostatic contacts; (2) although the percentage of base pairs in the A-form 
      is maintained in 5'UTR, the HCVc-120 protein is beta-sheet and beta-helix 
      enriched upon formation of protein-5'UTR macromolecular assemblies; (3) 
      protein-5'UTR interactions resulting in protein alpha-helix formation involve 
      guanine bases in duplex segments. The mentioned interactions may represent novel 
      targets for antiviral strategies against this important virus.
FAU - Rodriguez-Casado, Arantxa
AU  - Rodriguez-Casado A
AD  - Instituto de Estructura de la Materia (CSIC), Serrano 121, 28006 Madrid, Spain.
FAU - Molina, Marina
AU  - Molina M
FAU - Carmona, Pedro
AU  - Carmona P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Spectrosc
JT  - Applied spectroscopy
JID - 0372406
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - 5' Untranslated Regions/chemistry
MH  - Circular Dichroism/*methods
MH  - Hepacivirus/*chemistry
MH  - Hydrogen Bonding
MH  - RNA, Viral/*chemistry
MH  - Spectrum Analysis, Raman/*methods
MH  - Viral Core Proteins/*chemistry
EDAT- 2007/11/22 09:00
MHDA- 2007/12/19 09:00
CRDT- 2007/11/22 09:00
PHST- 2007/11/22 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/11/22 09:00 [entrez]
AID - 10.1366/000370207782597139 [doi]
PST - ppublish
SO  - Appl Spectrosc. 2007 Nov;61(11):1219-24. doi: 10.1366/000370207782597139.