PMID- 18029605
OWN - NLM
STAT- MEDLINE
DCOM- 20080428
LR  - 20071121
IS  - 1746-8272 (Electronic)
IS  - 0261-3166 (Linking)
IP  - 51
DP  - 2007
TI  - Aptameric enzyme subunit for homogeneous protein sensing.
PG  - 99-100
AB  - We have developed an aptameric enzyme subunit (AES) for protein sensing. AES is 
      composed of an enzyme-inhibiting aptamer and a target molecule-binding aptamer 
      and it can allosterically control the corresponding enzyme activity by binding 
      target molecules. Here, we applied AES to immunoglobulin E (IgE) sensing. We 
      split the thrombin-inhibiting aptamer into two oligonucleotides and then 
      connected IgE-binding aptamer and its complementary strand to the split 
      thrombin-inhibiting aptamer. The hybrid of these two oligonucleotides inhibited 
      thrombin enzymatic activity and it was decreased in the presence of IgE. We were 
      able to detect IgE by using the AES in homogeneous assay and detection limit was 
      250 nM.
FAU - Yoshida, Wataru
AU  - Yoshida W
AD  - Department of Biotechnology, Graduate School of Engineering, Tokyo University of 
      Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
FAU - Sode, Koji
AU  - Sode K
FAU - Ikebukuro, Kazunori
AU  - Ikebukuro K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Symp Ser (Oxf)
JT  - Nucleic acids symposium series (2004)
JID - 101259965
RN  - 0 (Aptamers, Nucleotide)
RN  - 145563-68-4 (thrombin aptamer)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Base Sequence
MH  - Immunoglobulin E/*analysis
MH  - Molecular Sequence Data
MH  - Thrombin/antagonists & inhibitors
EDAT- 2007/11/22 09:00
MHDA- 2008/04/29 09:00
CRDT- 2007/11/22 09:00
PHST- 2007/11/22 09:00 [pubmed]
PHST- 2008/04/29 09:00 [medline]
PHST- 2007/11/22 09:00 [entrez]
AID - 51/1/99 [pii]
AID - 10.1093/nass/nrm050 [doi]
PST - ppublish
SO  - Nucleic Acids Symp Ser (Oxf). 2007;(51):99-100. doi: 10.1093/nass/nrm050.