PMID- 18031005
OWN - NLM
STAT- MEDLINE
DCOM- 20080205
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 55
IP  - 25
DP  - 2007 Dec 12
TI  - Online YPA4 resin microcolumn separation/preconcentration coupled with 
      inductively coupled plasma optical emission spectrometry (ICP-OES) for the 
      speciation analysis of mercury in seafood.
PG  - 10129-34
AB  - A simple and effective method for the determination of trace amounts of 
      methylmercury (MeHg(+)) and inorganic mercury (Hg(2+)) in seafood was developed 
      by online microcolumn separation/preconcentration combined with inductively 
      coupled plasma optical emission spectrometry (ICP-OES). It was found that Hg(2+) 
      could be quantitatively adsorbed by YPA 4 resin from pH 7.0 to strong acidic 
      medium (6 mol L(-1) HCl) and that MeHg(+) was retained by the YPA 4 microcolumn 
      only at pH 1.0-7.0. Therefore, a strong acidic medium (about 5 mol L(-1) HCl), 
      which could liberate mercury species from biological samples, was used to 
      directly separate inorganic Hg(2+) from total Hg, and MeHg(+) in effluent was 
      retained by YPA 4 column after the effluent was adjusted to pH 1.5. The effects 
      of acidity, sample flow rate and volume, elution solution, and interfering ions 
      on recovery of the two mercury species have been systematically investigated. 
      Under optimal conditions, the limits of detection (LODs) were 72 and 44 ng L(-1) 
      for Hg(2+) and MeHg(+) (as Hg) with online concentration factors of 12.5 and 
      12.1, respectively. The relative standard deviations (RSDs) for nine replicate 
      determinations at 5 ng mL(-1) levels of mercury species were 2.7 and 2.0% for 
      Hg(2+) and MeHg(+), respectively. The calibration graphs were linear with a 
      correlation coefficient of 0.9902 in the range of 0.5-100 ng mL(-1) for Hg(2+) 
      and 0.9976 in the range of 0.1-100 ng mL(-1) for MeHg(+), respectively. The 
      developed method was successfully applied to the direct determination of MeHg(+) 
      and Hg(2+) in seafood samples, and the recoveries for the spiked samples were in 
      the range of 89.9-102.4% (MeHg(+)) and 87.0-104.6% (Hg(2+)), respectively. The 
      method was validated by analyzing a certified reference material DORM-2 (dogfish 
      muscle), and the determined values were in good agreement with certified values.
FAU - Xiong, Chaomei
AU  - Xiong C
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of 
      China.
FAU - Hu, Bin
AU  - Hu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071122
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Cations, Divalent)
RN  - 0 (Chelating Agents)
RN  - 0 (Methylmercury Compounds)
RN  - FXS1BY2PGL (Mercury)
RN  - GYV9AM2QAG (Thiourea)
SB  - IM
MH  - Cations, Divalent/analysis
MH  - Chelating Agents
MH  - Chromatography/methods
MH  - Hydrogen-Ion Concentration
MH  - Mercury/*analysis
MH  - Methylmercury Compounds/analysis
MH  - Seafood/*analysis
MH  - Spectrum Analysis/methods
MH  - Thiourea
EDAT- 2007/11/23 09:00
MHDA- 2008/02/06 09:00
CRDT- 2007/11/23 09:00
PHST- 2007/11/23 09:00 [pubmed]
PHST- 2008/02/06 09:00 [medline]
PHST- 2007/11/23 09:00 [entrez]
AID - 10.1021/jf071979b [doi]
PST - ppublish
SO  - J Agric Food Chem. 2007 Dec 12;55(25):10129-34. doi: 10.1021/jf071979b. Epub 2007 
      Nov 22.