PMID- 18031354
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20190524
IS  - 1601-5223 (Electronic)
IS  - 0018-0661 (Linking)
VI  - 144
IP  - 5
DP  - 2007 Nov
TI  - Involvement of co-repressors Groucho and CtBP in the regulation of single-minded 
      in Drosophila.
PG  - 195-205
AB  - Dorso-ventral patterning results in the establishment of the two germ layers in 
      the Drosophila embryo, mesoderm and mesectoderm, that are separated by a strip of 
      cells giving rise to the mesectoderm and eventually to the ventral midline. The 
      mesectoderm is specified by the expression of single-minded (sim) which is 
      activated through the concerted action of Dorsal and Twist in addition to a Notch 
      signal. In the mesoderm, sim is repressed by Snail together with the co-repressor 
      C-terminal binding protein (CtBP). Here, we address the involvement of the two 
      co-repressors CtBP and Groucho (Gro) in repression of sim in the neuroectoderm. 
      It was shown earlier that sim is restricted in the neuroectoderm with help of 
      Suppressor of Hairless [Su(H)] and Hairless. Using the female sterile technique, 
      we generated germ line clones deficient for Gro, CtBP or Hairless and assayed sim 
      mRNA relative to snail mRNA expression. We show that sim repression requires both 
      co-repressors Gro and CtBP to be fully repressed in the neuroectoderm, suggesting 
      that a repression complex is assembled including Su(H) and Hairless as was shown 
      for other Notch target genes before. Moreover, our work implies that Gro is 
      important for the repression of sim specifically within the mesoderm anlagen, 
      indicating that Snail and CtBP are insufficient to entirely silence sim in this 
      germ layer.
FAU - Nagel, Anja C
AU  - Nagel AC
AD  - Universitat Hohenheim, Institut fur Genetik, Stuttgart, Germany.
FAU - Wech, Irmgard
AU  - Wech I
FAU - Schwinkendorf, Daniela
AU  - Schwinkendorf D
FAU - Preiss, Anette
AU  - Preiss A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (Su(H) protein, Drosophila)
RN  - 0 (Transcription Factors)
RN  - 0 (gro protein, Drosophila)
RN  - 0 (sim protein, Drosophila)
RN  - 0 (sna protein, Drosophila)
RN  - 148349-66-0 (H protein, Drosophila)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.- (C-terminal binding protein)
SB  - IM
MH  - Alcohol Oxidoreductases/*physiology
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism/*physiology
MH  - DNA-Binding Proteins/*physiology
MH  - Drosophila/*embryology
MH  - Drosophila Proteins/genetics/*metabolism/physiology
MH  - Neural Plate/metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Repressor Proteins/*physiology
MH  - Snail Family Transcription Factors
MH  - Transcription Factors/physiology
EDAT- 2007/11/23 09:00
MHDA- 2007/12/19 09:00
CRDT- 2007/11/23 09:00
PHST- 2007/11/23 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/11/23 09:00 [entrez]
AID - HRD2020 [pii]
AID - 10.1111/j.2007.0018-0661.02020.x [doi]
PST - ppublish
SO  - Hereditas. 2007 Nov;144(5):195-205. doi: 10.1111/j.2007.0018-0661.02020.x.