PMID- 18034759
OWN - NLM
STAT- MEDLINE
DCOM- 20080324
LR  - 20181201
IS  - 1750-3841 (Electronic)
IS  - 0022-1147 (Linking)
VI  - 72
IP  - 9
DP  - 2007 Nov
TI  - Antiallergic activities of pigmented rice bran extracts in cell assays.
PG  - S719-26
AB  - Using a panel of chemical, biochemical, and cell assays, we determined inhibitory 
      effects of extracts of the pigmented black rice brans on in vitro allergic 
      reactions. Ethanol-water (70% v/v) extracts from 5 pigmented brans were found to 
      be more effective than an extract from a nonpigmented rice cultivar in 
      suppressing the release of histamine and beta-hexosaminidase from basophilic 
      RBL-2H3 cells stimulated with both Ionophore A23187 and immunoglobulin E 
      (IgE)-antigen complexes. Suppression was also obtained with A23187-stimulated rat 
      peritoneal mast cells. The extent of inhibition of these 2 markers of the immune 
      response was accompanied by an influx of calcium ions. The inhibition of the 
      immune process by the pigmented brans was confirmed by the observed modulation of 
      the proinflammatory cytokine gene expressions and cytokine release, as indicated 
      by the reduction in tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, 
      IL-4, and IL-6 mRNA expressions determined with the reverse 
      transcription-polymerase chain reaction (RT-PCR). Reduction of TNF-alpha, 
      IL-1beta, and IL-6 protein release from both the cultured cell line and 
      peritoneal cells was further confirmed by enzyme-linked immunoadsorbent assays. 
      Rice bran from the LK1-3-6-12-1-1 cultivar was the most effective inhibitor in 
      all assays. This particular rice variety merits further evaluation as part of a 
      human diet to ascertain its potential to protect against allergic diseases such 
      as hay fever and asthma.
FAU - Choi, Sun Phil
AU  - Choi SP
AD  - Dept. of Biological Science, Ajou Univ., Suwon 443-749, Korea.
FAU - Kang, Mi Young
AU  - Kang MY
FAU - Koh, Hee Jong
AU  - Koh HJ
FAU - Nam, Seok Hyun
AU  - Nam SH
FAU - Friedman, Mendel
AU  - Friedman M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Sci
JT  - Journal of food science
JID - 0014052
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Interleukins)
RN  - 0 (Pigments, Biological)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 820484N8I3 (Histamine)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/chemistry/*pharmacology
MH  - Calcium/immunology/metabolism
MH  - Cell Line, Tumor
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression/drug effects/immunology
MH  - Histamine/metabolism
MH  - Histamine Release/drug effects/immunology
MH  - Interleukins/metabolism
MH  - Male
MH  - Mast Cells/*drug effects/immunology/metabolism
MH  - Oryza/*chemistry/genetics
MH  - Phytotherapy
MH  - *Pigments, Biological
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seeds/immunology
MH  - Species Specificity
MH  - Tumor Necrosis Factor-alpha/drug effects/metabolism
MH  - beta-N-Acetylhexosaminidases/drug effects/metabolism
EDAT- 2007/11/24 09:00
MHDA- 2008/03/25 09:00
CRDT- 2007/11/24 09:00
PHST- 2007/11/24 09:00 [pubmed]
PHST- 2008/03/25 09:00 [medline]
PHST- 2007/11/24 09:00 [entrez]
AID - JFDS562 [pii]
AID - 10.1111/j.1750-3841.2007.00562.x [doi]
PST - ppublish
SO  - J Food Sci. 2007 Nov;72(9):S719-26. doi: 10.1111/j.1750-3841.2007.00562.x.