PMID- 18037262
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20080204
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 44
IP  - 3
DP  - 2008 Mar
TI  - Mutations in the periplasmic chaperone leading to loss of surface expression of 
      the colonization factor CS6 in enterotoxigenic Escherichia coli (ETEC) clinical 
      isolates.
PG  - 246-54
AB  - Enterotoxigenic Escherichia coli (ETEC) cause diarrhoea by adhesion to human 
      enterocytes by one or more colonization factors (CFs) and secretion of 
      heat-labile (LT) and/or heat-stable (ST) enterotoxins. Expression of coli surface 
      antigen 6 (CS6) on the bacterial surface, usually associated with ETEC strains 
      that produce ST alone or in combination with LT, is rarely found in strains 
      expressing only LT. However, a number of LT-only strains which are genotypically 
      positive but phenotypically negative for CS6 have been identified. In this study, 
      eight such strains from India and Guinea-Bissau belonging to different clones 
      were analysed. The CS6 operon cssABCD was transcribed but protein analyses 
      suggested that the structural subunits CssA and CssB of CS6 were absent in the 
      periplasm. Most strains contained truncating mutations within the periplasmic 
      chaperone-encoding gene cssC and protein modelling indicated that this severely 
      affected the substrate-binding capacity of the chaperone. A single-nucleotide 
      polymorphism (SNP) (A-->T) in the 5'-untranslated region of cssC distinguished 
      the eight strains from ETEC strains that do express CS6 on the surface and may be 
      a potential marker for ETEC strains containing phenotypically silent cssABCD. The 
      study emphasizes the importance of using both genotypic and phenotypic methods in 
      epidemiological studies of ETEC, e.g. for vaccine development.
FAU - Nicklasson, Matilda
AU  - Nicklasson M
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, Goteborg 
      University, P.O. Box 435, 405 30 Goteborg, Sweden. 
      matilda.nicklasson@microbio.gu.se
FAU - Sjoling, Asa
AU  - Sjoling A
FAU - Lebens, Michael
AU  - Lebens M
FAU - Tobias, Joshua
AU  - Tobias J
FAU - Janzon, Anders
AU  - Janzon A
FAU - Brive, Lars
AU  - Brive L
FAU - Svennerholm, Ann-Mari
AU  - Svennerholm AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071018
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antigens, Bacterial)
RN  - 0 (CS6 antigen, E coli)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Periplasmic Proteins)
SB  - IM
MH  - Antigens, Bacterial/analysis/genetics/*metabolism
MH  - Enterotoxigenic Escherichia coli/genetics/immunology/*metabolism
MH  - Enterotoxins
MH  - Escherichia coli Infections/epidemiology/microbiology
MH  - Escherichia coli Proteins/analysis/genetics/*metabolism
MH  - Molecular Chaperones/genetics
MH  - Mutation/*genetics
MH  - Operon
MH  - Periplasmic Proteins/metabolism
MH  - Polymorphism, Single Nucleotide
EDAT- 2007/11/27 09:00
MHDA- 2008/05/31 09:00
CRDT- 2007/11/27 09:00
PHST- 2007/03/30 00:00 [received]
PHST- 2007/06/11 00:00 [accepted]
PHST- 2007/11/27 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2007/11/27 09:00 [entrez]
AID - S0882-4010(07)00136-2 [pii]
AID - 10.1016/j.micpath.2007.06.009 [doi]
PST - ppublish
SO  - Microb Pathog. 2008 Mar;44(3):246-54. doi: 10.1016/j.micpath.2007.06.009. Epub 
      2007 Oct 18.