PMID- 18037790
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20190823
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 51
IP  - 11
DP  - 2007
TI  - Novel functions of herbal medicines in dendritic cells: role of Amomi Semen in 
      tumor immunity.
PG  - 1121-33
AB  - Dendritic cells (DCs) have a major role in regulating immune responses, including 
      tumor immunity and peripheral tolerance. In the present study, we identified 
      novel functions of herbal medicines in DCs by screening 99 herbal medicines, most 
      of which are among the 210 Chinese medicines approved by the Ministry of Health, 
      Labour, and Welfare, Japan. Ethanol extracts were prepared, and a murine 
      epidermal-derived Langerhans cell line, XS106, was used to screen the 99 extracts 
      by analyzing major histocompatibility complex (MHC) class II expression. Amomi 
      Semen (amomum seed), Polyporus (polyporus sclerotium), and Plantaginis Semen 
      (plantago seed) potently activated XS106 and were selected for further analysis. 
      The effects of these extracts on bone marrow-derived DCs (BM-DCs) generated in 
      vitro were then analyzed using surface phenotype (MHC class II, CD80, and CD86) 
      and interleukin (IL)-12p70 production as indicators. BM-DCs treated with Amomi 
      Semen extract exhibited activated phenotypes and secreted IL-12p70. The 
      activation level was similar to that induced by lipopolysaccharides. Finally, an 
      E.G7-OVA tumor model (E.L4-OVA transfectant) was used to examine the anti-tumor 
      effects of Amomi Semen extract. Vaccination of mice with a subcutaneous injection 
      of BM-DCs treated with Amomi Semen extract and OVA peptide significantly 
      inhibited the growth of tumor cells and prolonged survival time compared to 
      controls. Furthermore, therapeutic effects were observed on established tumors. 
      The inhibition rates for both the prophylactic and therapeutic protocols were 
      comparable to those of lipopolysaccharides. These results indicate that Amomi 
      Semen extract potently activate DCs and is potentially useful for DC vaccination.
FAU - Fukui, Hajime
AU  - Fukui H
AD  - Department of Geriatric and Environmental Dermatology, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, Japan.
FAU - Mitsui, Seika
AU  - Mitsui S
FAU - Harima, Nobue
AU  - Harima N
FAU - Nose, Mitsuhiko
AU  - Nose M
FAU - Tsujimura, Kunio
AU  - Tsujimura K
FAU - Mizukami, Hajime
AU  - Mizukami H
FAU - Morita, Akimichi
AU  - Morita A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Plant Extracts)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Amomum/*chemistry
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/immunology
MH  - Cell Line
MH  - Dendritic Cells/*drug effects/immunology
MH  - *Herbal Medicine
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/immunology
MH  - Plant Extracts/*pharmacology
MH  - Plants, Medicinal
EDAT- 2007/11/27 09:00
MHDA- 2008/05/31 09:00
CRDT- 2007/11/27 09:00
PHST- 2007/11/27 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2007/11/27 09:00 [entrez]
AID - JST.JSTAGE/mandi/51.1121 [pii]
AID - 10.1111/j.1348-0421.2007.tb03998.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 2007;51(11):1121-33. doi: 10.1111/j.1348-0421.2007.tb03998.x.