PMID- 18037985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211020
IS  - 1350-4487 (Print)
IS  - 1350-4487 (Linking)
VI  - 42
IP  - 6-7
DP  - 2007 Jul
TI  - Sample Tracking in an Automated Cytogenetic Biodosimetry Laboratory for Radiation 
      Mass Casualties.
PG  - 1119-1124
AB  - Chromosome aberration-based dicentric assay is expected to be used after mass 
      casualty life-threatening radiation exposures to assess radiation dose to 
      individuals. This will require processing of a large number of samples for 
      individual dose assessment and clinical triage to aid treatment decisions. We 
      have established an automated, high-throughput, cytogenetic biodosimetry 
      laboratory to process a large number of samples for conducting the dicentric 
      assay using peripheral blood from exposed individuals according to 
      internationally accepted laboratory protocols (i.e., within days following 
      radiation exposures). The components of an automated cytogenetic biodosimetry 
      laboratory include blood collection kits for sample shipment, a cell viability 
      analyzer, a robotic liquid handler, an automated metaphase harvester, a metaphase 
      spreader, high-throughput slide stainer and coverslipper, a high-throughput 
      metaphase finder, multiple satellite chromosome-aberration analysis systems, and 
      a computerized sample tracking system. Laboratory automation using commercially 
      available, off-the-shelf technologies, customized technology integration, and 
      implementation of a laboratory information management system (LIMS) for 
      cytogenetic analysis will significantly increase throughput.This paper focuses on 
      our efforts to eliminate data transcription errors, increase efficiency, and 
      maintain samples' positive chain-of-custody by sample tracking during sample 
      processing and data analysis. This sample tracking system represents a "beta" 
      version, which can be modeled elsewhere in a cytogenetic biodosimetry laboratory, 
      and includes a customized LIMS with a central server, personal computer 
      workstations, barcode printers, fixed station and wireless hand-held devices to 
      scan barcodes at various critical steps, and data transmission over a private 
      intra-laboratory computer network. Our studies will improve diagnostic 
      biodosimetry response, aid confirmation of clinical triage, and medical 
      management of radiation exposed individuals.
FAU - Martin, P R
AU  - Martin PR
AD  - Uniformed Services University of Health Sciences, Armed Forces Radiobiology 
      Research Institute, 8901 Wisconsin Avenue. Bethesda, MD 20889-5603, USA.
FAU - Berdychevski, R E
AU  - Berdychevski RE
FAU - Subramanian, U
AU  - Subramanian U
FAU - Blakely, W F
AU  - Blakely WF
FAU - Prasanna, P G S
AU  - Prasanna PG
LA  - eng
GR  - Y01 AI003823-01/AI/NIAID NIH HHS/United States
GR  - Y01 AI005045/AI/NIAID NIH HHS/United States
GR  - Y01 AI005045-01/AI/NIAID NIH HHS/United States
GR  - Y01 DK003508-01/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Radiat Meas
JT  - Radiation measurements
JID - 9890953
PMC - PMC2084199
MID - NIHMS32166
EDAT- 2007/11/27 09:00
MHDA- 2007/11/27 09:01
PMCR- 2008/07/01
CRDT- 2007/11/27 09:00
PHST- 2007/11/27 09:00 [pubmed]
PHST- 2007/11/27 09:01 [medline]
PHST- 2007/11/27 09:00 [entrez]
PHST- 2008/07/01 00:00 [pmc-release]
AID - 10.1016/j.radmeas.2007.05.021 [doi]
PST - ppublish
SO  - Radiat Meas. 2007 Jul;42(6-7):1119-1124. doi: 10.1016/j.radmeas.2007.05.021.