PMID- 18038419
OWN - NLM
STAT- MEDLINE
DCOM- 20080219
LR  - 20071126
IS  - 1932-6254 (Print)
IS  - 1932-6254 (Linking)
VI  - 1
IP  - 4
DP  - 2007 Jul-Aug
TI  - Spatial differences of cellular origins and in vivo hypoxia modify contractile 
      properties of pulmonary artery smooth muscle cells: lessons for arterial tissue 
      engineering.
PG  - 287-95
AB  - Tissue engineering of functional arteries is challenging. Within the pulmonary 
      artery wall, smooth muscle cells (PASMCs) have site-specific developmental and 
      functional phenotypes, reflecting differing contractile roles. The force 
      generated by PASMCs isolated from the inner 25% and outer 50% of the media of 
      intrapulmonary elastic arteries from five normal and eight chronically hypoxic 
      (hypertensive) 14 day-old piglets was quantified in a three-dimensional (3D) 
      collagen construct, using a culture force monitor. Outer medial PASMCs from 
      normal piglets exerted more force (528 +/- 50 dynes) than those of hypoxic 
      piglets (177 +/- 42 dynes; p < 0.01). Force generation by inner medial PASMCs 
      from normal and hypoxic piglets was similar (349 +/- 35 and 239 +/- 60 dynes). In 
      response to agonist (thromboxane) stimulation, all PASMCs from normal and hypoxic 
      piglets contracted, but the increase in force generated by outer and inner 
      hypoxic PASMCs (ranges 13-72 and 14-56 dynes) was less than by normal PASMCs 
      (ranges 27-154 and 34-159 dynes, respectively; p < 0.05 for both). All hypoxic 
      PASMCs were unresponsive to antagonist (sodium nitroprusside) stimulation, all 
      normal PASMCs relaxed (range - 87 to - 494 dynes). Myosin heavy chain expression 
      by both hypoxic PASMC phenotypes was less than normal (p < 0.05 for both), as was 
      the activity of focal adhesion kinase, regulating contraction, in hypoxic inner 
      PASMCs (p < 0.01). Chronic hypoxia resulted in the development of abnormal PASMC 
      phenotypes, which in collagen constructs exhibited a reduction in contractile 
      force and reactivity to agonists. Characterization of the mechanical response of 
      spatially distinct cells and modification of their behaviour by hypoxia is 
      critical for successful tissue engineering of major blood vessels.
FAU - Hall, S M
AU  - Hall SM
AD  - University College London, Institute of Child Health, 30 Guilford Street, London, 
      UK. s.hall@ich.ucl.ac.uk
FAU - Soueid, A
AU  - Soueid A
FAU - Smith, T
AU  - Smith T
FAU - Brown, R A
AU  - Brown RA
FAU - Haworth, S G
AU  - Haworth SG
FAU - Mudera, V
AU  - Mudera V
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Contractile Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Vasoconstrictor Agents)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Contractile Proteins/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - *Muscle Contraction/drug effects
MH  - Muscle Relaxation/drug effects
MH  - Myocytes, Smooth Muscle/*cytology/drug effects/metabolism
MH  - Pulmonary Artery/*cytology/drug effects/metabolism
MH  - Swine
MH  - *Tissue Engineering
MH  - Vasoconstrictor Agents/pharmacology
EDAT- 2007/11/27 09:00
MHDA- 2008/02/20 09:00
CRDT- 2007/11/27 09:00
PHST- 2007/11/27 09:00 [pubmed]
PHST- 2008/02/20 09:00 [medline]
PHST- 2007/11/27 09:00 [entrez]
AID - 10.1002/term.39 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2007 Jul-Aug;1(4):287-95. doi: 10.1002/term.39.