PMID- 18040027
OWN - NLM
STAT- MEDLINE
DCOM- 20080109
LR  - 20220408
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 116
IP  - 24
DP  - 2007 Dec 11
TI  - Cardioprotective effects of short-term caloric restriction are mediated by 
      adiponectin via activation of AMP-activated protein kinase.
PG  - 2809-17
AB  - BACKGROUND: Overeating and obesity are major health problems in developed 
      countries. Caloric restriction (CR) can counteract the deleterious aspects of 
      obesity-related diseases and prolong lifespan. We have demonstrated that 
      short-term CR improves myocardial ischemic tolerance and increases adiponectin 
      levels. Here, we investigated the specific role of adiponectin in CR-induced 
      cardioprotection. METHODS AND RESULTS: Adiponectin antisense transgenic (Ad-AS) 
      mice and wild-type (WT) mice were randomly assigned to a group fed ad libitum and 
      a CR group (90% of caloric intake of ad libitum for 3 weeks, then 65% for 2 
      weeks). Isolated perfused mouse hearts were subjected to 25 minutes of ischemia, 
      followed by 60 minutes of reperfusion. CR increased serum adiponectin levels by 
      84% in WT mice. Gel filtration analysis of the oligomeric complex distribution 
      showed that CR produced a marked increase in the high-molecular-weight complex of 
      adiponectin in WT mice; in contrast, CR did not change serum adiponectin levels 
      or their oligomeric pattern in Ad-AS mice. CR improved the recovery of left 
      ventricular function after ischemia/reperfusion and limited infarct size in WT 
      mice; these effects were completely abrogated in Ad-AS mice. CR also increased 
      the phosphorylated form of AMP-activated protein kinase and acetyl-CoA 
      carboxylase in WT but not in Ad-AS mice. Recombinant adiponectin restored 
      CR-induced cardioprotection in Ad-AS mice, and inhibition of AMP-activated 
      protein kinase phosphorylation completely abrogated CR-induced cardioprotection 
      in WT mice. CONCLUSIONS: The cardioprotective effects of short-term CR are 
      mediated by increased production of adiponectin and the associated activation of 
      AMP-activated protein kinase.
FAU - Shinmura, Ken
AU  - Shinmura K
AD  - Division of Geriatric Medicine, Department of Internal Medicine, Keio University 
      School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan 160-8582. 
      shimmura@sc.itc.keio.ac.jp
FAU - Tamaki, Kayoko
AU  - Tamaki K
FAU - Saito, Kiyomi
AU  - Saito K
FAU - Nakano, Yasuko
AU  - Nakano Y
FAU - Tobe, Takashi
AU  - Tobe T
FAU - Bolli, Roberto
AU  - Bolli R
LA  - eng
GR  - HL-55757/HL/NHLBI NIH HHS/United States
GR  - HL-70897/HL/NHLBI NIH HHS/United States
GR  - HL-76794/HL/NHLBI NIH HHS/United States
GR  - HL-78825/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071126
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Adiponectin)
RN  - EC 2.7.4.3 (Adenylate Kinase)
SB  - IM
CIN - Circulation. 2007 Dec 11;116(24):2779-81. PMID: 18071087
MH  - Adenylate Kinase/*metabolism
MH  - Adiponectin/*physiology
MH  - Animals
MH  - *Diet, Reducing
MH  - Disease Models, Animal
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - Myocardial Ischemia/*diet therapy/enzymology/*physiopathology
MH  - Obesity/prevention & control
EDAT- 2007/11/28 09:00
MHDA- 2008/01/10 09:00
CRDT- 2007/11/28 09:00
PHST- 2007/11/28 09:00 [pubmed]
PHST- 2008/01/10 09:00 [medline]
PHST- 2007/11/28 09:00 [entrez]
AID - CIRCULATIONAHA.107.725697 [pii]
AID - 10.1161/CIRCULATIONAHA.107.725697 [doi]
PST - ppublish
SO  - Circulation. 2007 Dec 11;116(24):2809-17. doi: 10.1161/CIRCULATIONAHA.107.725697. 
      Epub 2007 Nov 26.