PMID- 18040049
OWN - NLM
STAT- MEDLINE
DCOM- 20080118
LR  - 20220223
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 104
IP  - 49
DP  - 2007 Dec 4
TI  - Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1.
PG  - 19464-9
AB  - Dendritic cells (DCs) efficiently capture HIV-1 and mediate transmission to T 
      cells, but the underlying molecular mechanism is still being debated. The C-type 
      lectin DC-SIGN is important in HIV-1 transmission by DCs. However, various 
      studies strongly suggest that another HIV-1 receptor on DCs is involved in the 
      capture of HIV-1. Here we have identified syndecan-3 as a major HIV-1 attachment 
      receptor on DCs. Syndecan-3 is a DC-specific heparan sulfate (HS) proteoglycan 
      that captures HIV-1 through interaction with the HIV-1 envelope glycoprotein 
      gp120. Syndecan-3 stabilizes the captured virus, enhances DC infection in cis, 
      and promotes transmission to T cells. Removal of the HSs from the cell surface by 
      heparinase III or by silencing syndecan-3 by siRNA partially inhibited HIV-1 
      transmission by immature DCs, whereas neutralizing both syndecan-3 and DC-SIGN 
      completely abrogated HIV-1 capture and subsequent transmission. Thus, HIV-1 
      exploits both syndecan-3 and DC-SIGN to mediate HIV-1 transmission, and an 
      effective microbicide should target both syndecan-3 and DC-SIGN on DCs to prevent 
      transmission.
FAU - de Witte, Lot
AU  - de Witte L
AD  - Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, 
      USA.
FAU - Bobardt, Michael
AU  - Bobardt M
FAU - Chatterji, Udayan
AU  - Chatterji U
FAU - Degeest, Gisele
AU  - Degeest G
FAU - David, Guido
AU  - David G
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TB
FAU - Gallay, Philippe
AU  - Gallay P
LA  - eng
GR  - R21 AI071952/AI/NIAID NIH HHS/United States
GR  - R33 AI071952/AI/NIAID NIH HHS/United States
GR  - R33 AI076005/AI/NIAID NIH HHS/United States
GR  - AI 071952/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20071126
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, HIV)
RN  - 0 (Syndecan-3)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Dendritic Cells/*immunology/virology
MH  - *HIV-1
MH  - Humans
MH  - RNA, Small Interfering/pharmacology
MH  - Receptors, HIV/antagonists & inhibitors/genetics/*metabolism
MH  - Syndecan-3/antagonists & inhibitors/genetics/*metabolism
MH  - T-Lymphocytes/*immunology/virology
MH  - *Virus Internalization
PMC - PMC2148312
COIS- The authors declare no conflict of interest.
EDAT- 2007/11/28 09:00
MHDA- 2008/01/19 09:00
PMCR- 2008/06/04
CRDT- 2007/11/28 09:00
PHST- 2007/11/28 09:00 [pubmed]
PHST- 2008/01/19 09:00 [medline]
PHST- 2007/11/28 09:00 [entrez]
PHST- 2008/06/04 00:00 [pmc-release]
AID - 0703747104 [pii]
AID - 8220 [pii]
AID - 10.1073/pnas.0703747104 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19464-9. doi: 
      10.1073/pnas.0703747104. Epub 2007 Nov 26.