PMID- 18040070
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20151119
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 232
IP  - 11
DP  - 2007 Dec
TI  - Nitric oxide bioavailability and not production is first altered during the onset 
      of insulin resistance in sucrose-fed rats.
PG  - 1458-64
AB  - Although the role of nitric oxide (NO) in peripheral glucose uptake has been 
      thoroughly described, little is known regarding the alterations in NO metabolism 
      during the early onset of insulin resistance. During this study we investigated 
      the alterations in NO synthesis and bioavailability in a model for dietary 
      modulations of insulin sensitivity. For 6 weeks, rats were fed a standard diet 
      (C), a high-sucrose diet inducing insulin resistance (HS), or high-sucrose diets 
      supplemented with cysteine, which endowed protection against the 
      high-sucrose-induced insulin resistance (Ti). Several markers of NO synthesis and 
      bioavailability were assessed and confronted with markers of insulin sensitivity. 
      After 5 weeks, although urinary cGMP excretion did not differ between the groups, 
      insulin resistance in HS rats was associated with both a significant increase in 
      NO oxidation, as determined by plasma nitrotyrosine concentrations, and in the 
      inducible NO synthase (iNOS)/endothelial NO synthase (iNOS/eNOS) mRNA ratio in 
      skeletal muscle compared with C rats. These alterations were prevented in rats 
      fed the cysteine-rich diets. NO production, as assessed by urinary 15NO3* 
      excretion following a [15N2-(guanido)]-arginine intra-venous bolus, independently 
      and significantly correlated with insulin sensitivity but did not significantly 
      differ between C, HS, and Ti rats; neither did the aortic eNOS protein expression 
      or skeletal muscle insulin-induced eNOS activation. Our results indicate that in 
      this model of dietary modulations of insulin sensitivity (i) NO production 
      accounts for part of total inter-individual variation in insulin sensitivity, but 
      (ii) early diet-related changes in insulin sensitivity are accompanied by changes 
      in NO bioavailability.
FAU - Blouet, Clemence
AU  - Blouet C
AD  - INRA, AgroParisTech, UMR914 Nutrition Physiology and Ingestive Behavior, Centre 
      de Recherche en Nutrition Humaine-Ile de France, 75005 Paris, France.
FAU - Mariotti, Francois
AU  - Mariotti F
FAU - Mathe, Veronique
AU  - Mathe V
FAU - Tome, Daniel
AU  - Tome D
FAU - Huneau, Jean-Francois
AU  - Huneau JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Biomarkers)
RN  - 0 (Nitrates)
RN  - 0 (Sweetening Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3604-79-3 (3-nitrotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - 57-50-1 (Sucrose)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - H2D2X058MU (Cyclic GMP)
RN  - K848JZ4886 (Cysteine)
SB  - IM
EIN - Exp Biol Med (Maywood). 2008 Apr;233(4):492
MH  - Animals
MH  - Aorta/enzymology
MH  - Biomarkers/blood/urine
MH  - Cyclic GMP/urine
MH  - Cysteine/pharmacology
MH  - Diet
MH  - Diet Therapy
MH  - Disease Models, Animal
MH  - Enzyme Activation/drug effects
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - *Insulin Resistance
MH  - Male
MH  - Muscle, Skeletal/enzymology
MH  - Muscle, Smooth, Vascular/enzymology
MH  - Nitrates/urine
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/*biosynthesis
MH  - Nitric Oxide Synthase Type III
MH  - Rats
MH  - Rats, Wistar
MH  - Sucrose/pharmacology/*toxicity
MH  - Sweetening Agents/pharmacology/*toxicity
MH  - Tyrosine/analogs & derivatives/blood
EDAT- 2007/11/28 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/11/28 09:00
PHST- 2007/11/28 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/11/28 09:00 [entrez]
AID - 232/11/1458 [pii]
AID - 10.3181/0703-RM-64 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2007 Dec;232(11):1458-64. doi: 10.3181/0703-RM-64.