PMID- 18040289
OWN - NLM
STAT- MEDLINE
DCOM- 20080401
LR  - 20071213
IS  - 1748-7838 (Electronic)
IS  - 1001-0602 (Linking)
VI  - 17
IP  - 12
DP  - 2007 Dec
TI  - A highly homozygous and parthenogenetic human embryonic stem cell line derived 
      from a one-pronuclear oocyte following in vitro fertilization procedure.
PG  - 999-1007
AB  - Homozygous human embryonic stem cells (hESCs) are thought to be better cell 
      sources for hESC banking because their human leukocyte antigen (HLA) haplotype 
      would strongly increase the degree of matching for certain populations with 
      relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from 
      parthenogenetic embryos, but only heterozygous hESCs have been established using 
      the current strategy to artificially activate the oocyte without second polar 
      body extrusion. Here we report the first successful derivation of a human 
      homozygous ESC line (chHES-32) from a one-pronuclear oocyte following routine in 
      vitro fertilization treatment. chHES-32 cells express common markers and genes 
      with normal hESCs. They have been propagated in an undifferentiated state for 
      more than a year (>P50) and have maintained a stable karyotype of 46, XX. When 
      differentiated in vivo and in vitro, chHES-32 cells can form derivatives from all 
      three embryonic germ layers. The almost undetectable expression of five 
      paternally expressed imprinted genes and their HLA genotype identical to the 
      oocyte donor indicated their parthenogenetic origin. Using genome-wide 
      single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity 
      of chHES-32 cells was further confirmed. The results indicated that 'unwanted' 
      one-pronuclear oocytes might be a potential source for human homozygous and 
      parthenogenetic ESCs, and suggested an alternative strategy for obtaining 
      homozygous hESC lines from parthenogenetic haploid oocytes.
FAU - Lin, Ge
AU  - Lin G
AD  - Institute of Reproductive and Stem Cell Engineering, Central South University, 
      Changsha 410078, China.
FAU - OuYang, Qi
AU  - OuYang Q
FAU - Zhou, Xiaoying
AU  - Zhou X
FAU - Gu, Yifan
AU  - Gu Y
FAU - Yuan, Ding
AU  - Yuan D
FAU - Li, Wen
AU  - Li W
FAU - Liu, Gang
AU  - Liu G
FAU - Liu, Tiancheng
AU  - Liu T
FAU - Lu, Guangxiu
AU  - Lu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Res
JT  - Cell research
JID - 9425763
RN  - 0 (HLA Antigens)
SB  - IM
CIN - Cell Res. 2008 Feb;18(2):215-7. PMID: 18246085
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line/*cytology/metabolism
MH  - Cell Lineage
MH  - Embryonic Stem Cells/*cytology/metabolism
MH  - *Fertilization in Vitro
MH  - Genomic Imprinting
MH  - HLA Antigens/metabolism
MH  - *Homozygote
MH  - Humans
MH  - Immunohistochemistry
MH  - Karyotyping
MH  - Mice
MH  - Mice, SCID
MH  - Oocytes/*cytology
MH  - *Parthenogenesis
MH  - Polymorphism, Single Nucleotide
EDAT- 2007/11/28 09:00
MHDA- 2008/04/02 09:00
CRDT- 2007/11/28 09:00
PHST- 2007/11/28 09:00 [pubmed]
PHST- 2008/04/02 09:00 [medline]
PHST- 2007/11/28 09:00 [entrez]
AID - cr200797 [pii]
AID - 10.1038/cr.2007.97 [doi]
PST - ppublish
SO  - Cell Res. 2007 Dec;17(12):999-1007. doi: 10.1038/cr.2007.97.