PMID- 18043040
OWN - NLM
STAT- MEDLINE
DCOM- 20080201
LR  - 20151119
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 31
IP  - 12
DP  - 2007 Dec
TI  - Urothelial carcinoma with an inverted growth pattern can be distinguished from 
      inverted papilloma by fluorescence in situ hybridization, immunohistochemistry, 
      and morphologic analysis.
PG  - 1861-7
AB  - Inverted papilloma of the urinary bladder and urothelial carcinoma with an 
      inverted (endophytic) growth pattern may be difficult to distinguish 
      histologically, especially in small biopsies. The distinction is important as 
      these lesions have very different biologic behaviors and are treated differently. 
      We examined histologic features and undertook immunohistochemical staining and 
      UroVysion fluorescence in situ hybridization (FISH) to determine whether these 
      methods could aid in making this distinction. We examined histologic sections 
      from 15 inverted papillomas and 29 urothelial carcinomas with an inverted growth 
      pattern. Each tumor was stained with antibodies to Ki-67, p53, and cytokeratin 
      20. In addition, each tumor was examined with UroVysion FISH for gains of 
      chromosomes 3, 7, and 17 and for loss of chromosome 9p21 signals. None of the 
      inverted papillomas stained positively for Ki-67 or for cytokeratin 20. Only 1 of 
      15 inverted papillomas stained positively for p53. By contrast, 66%, 59%, and 59% 
      of urothelial carcinomas with an inverted growth pattern stained positively for 
      Ki-67, p53, and cytokeratin 20, respectively. Only 3 of the urothelial carcinomas 
      stained negatively for all 3 immunohistochemical markers. UroVysion FISH produced 
      normal results for all cases of inverted papilloma. By contrast, 21 of 29 cases 
      (72%) of urothelial carcinoma with an inverted growth pattern demonstrated 
      chromosomal abnormalities typical of urothelial cancer and were considered 
      positive by UroVysion FISH criteria. Morphologic features, as well as 
      immunohistochemical stains (including stains for Ki-67, p53, and cytokeratin 20) 
      and/or UroVysion FISH can help to distinguish inverted papilloma from urothelial 
      carcinoma with an inverted growth pattern.
FAU - Jones, Timothy D
AU  - Jones TD
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN 46202, USA.
FAU - Zhang, Shaobo
AU  - Zhang S
FAU - Lopez-Beltran, Antonio
AU  - Lopez-Beltran A
FAU - Eble, John N
AU  - Eble JN
FAU - Sung, Ming-Tse
AU  - Sung MT
FAU - MacLennan, Gregory T
AU  - MacLennan GT
FAU - Montironi, Rodolfo
AU  - Montironi R
FAU - Tan, Puay-Hoon
AU  - Tan PH
FAU - Zheng, Suqin
AU  - Zheng S
FAU - Baldridge, Lee Ann
AU  - Baldridge LA
FAU - Cheng, Liang
AU  - Cheng L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Keratin-20)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Transitional Cell/metabolism/*pathology
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Keratin-20/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Middle Aged
MH  - Papilloma, Inverted/metabolism/*pathology
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Urinary Bladder Neoplasms/metabolism/*pathology
EDAT- 2007/11/29 09:00
MHDA- 2008/02/02 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2008/02/02 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - 00000478-200712000-00009 [pii]
AID - 10.1097/PAS.0b013e318060cb9d [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2007 Dec;31(12):1861-7. doi: 10.1097/PAS.0b013e318060cb9d.