PMID- 18043285
OWN - NLM
STAT- MEDLINE
DCOM- 20080114
LR  - 20220330
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 16
IP  - 4
DP  - 2007 Dec
TI  - Molecular differentiation of early and late stage laryngeal squamous cell 
      carcinoma: an exploratory analysis.
PG  - 218-21
AB  - BACKGROUND: A current shortcoming in cancer prognostication and treatment is a 
      lack of methods that adequately address the complexity and diversity of the 
      disease. Prognostic marker systems based on single parameters have generally 
      proven inadequate. Thus, multiparametric methods, which rely on many pieces of 
      information, are ideally suited to the grouping of tumor subtypes and the 
      identification of specific patterns of disease progression. DESIGN: This study 
      investigated, on an exploratory basis, whether genome wide alterations of loss 
      and gain, using a panel of 122 gene probes (112 unique genes), discriminated 
      between early stage (stage 1 and 2) and late stage (stage 3 and 4) laryngeal 
      squamous cell carcinomas (LSCC). The LSCC cohort comprised 29 patients, 12 early 
      and 17 late staged. Formalin-fixed LSCC DNA was interrogated by a genome wide 
      candidate gene panel (122 genes) using the multiplex ligation-dependent probe 
      amplification assay. RESULTS: Statistical analysis employed the nonparametric 
      Wilcoxon 2-sample test. Significant differences between tumor stages of early 
      versus late were seen for the following genes: ERBB4, CASP2, RECQL4, and BCL7A. 
      Loss of ERBB4 (P=0.045) and BCL7A (P=0.019) significantly discriminated between 
      early and late stage LSCC. Gain of RECQL4 copy number (P=0.043) was associated 
      with late LSCC. Gain of CASP2 (P=0.043) marked early LSCC, whereas loss was 
      associated with late LSCC. CONCLUSIONS: High-throughput genome wide approaches 
      have the potential to yield discrete gene repertoires of early and late stage 
      LSCC differentiation.
FAU - Saglam, Ozlen
AU  - Saglam O
AD  - Department of Pathology, Henry Ford Hospital, Detroit, MI, USA. 
      osaglam@hotmail.com
FAU - Shah, Veena
AU  - Shah V
FAU - Worsham, Maria J
AU  - Worsham MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Cohort Studies
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
EDAT- 2007/11/29 09:00
MHDA- 2008/01/15 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2008/01/15 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - 00019606-200712000-00005 [pii]
AID - 10.1097/PDM.0b013e3180d0aab5 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2007 Dec;16(4):218-21. doi: 10.1097/PDM.0b013e3180d0aab5.