PMID- 18043627
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20220310
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 1
IP  - 2
DP  - 2007 Jun
TI  - Parenteral long-acting amoxicillin reduces intestinal bacterial community 
      diversity in piglets even 5 weeks after the administration.
PG  - 180-3
AB  - We investigated the long-term effects of a single intramuscular administration of 
      amoxicillin (15 mg kg(-1)) 1 day after birth, on piglet intestinal microbiota. 
      Animals received no creep feed before weaning on day 28 of age. For the next 11 
      days, the piglets received a wheat-barley-based diet. Colon digesta samples were 
      collected on day 39 and subjected to denaturing gradient gel electrophoresis 
      (DGGE) of PCR-amplified 16S rRNA gene fragments. DGGE fingerprint diversity 
      indices differed between the group treated with amoxicillin and the untreated 
      group (0.8+/-0.19 and 1.03+/-0.17, respectively, P=0.012). Reamplification and 
      sequencing of two bands present in all samples revealed that a Roseburia 
      faecalis-related population was strongly reduced in relative abundance (98% 
      identity) in the treated group, while an enterobacterial population with 100% 
      identity to Shigella spp., Escherichia coli and Salmonella enterica serovar Typhi 
      was enriched. A band corresponding to Lactobacillus sobrius was present only in 
      the control group. The protective effect of prophylactic antibiotic 
      administration may be outweighed by the long-lasting disturbance of the gut 
      ecosystem.
FAU - Janczyk, Pawel
AU  - Janczyk P
AD  - Research Unit for Nutritional Physiology Oskar Kellner, Research Institute for 
      the Biology of Farm Animals, Dummerstorf, Germany.
FAU - Pieper, Robert
AU  - Pieper R
FAU - Souffrant, Wolfgang Bernhard
AU  - Souffrant WB
FAU - Bimczok, Diane
AU  - Bimczok D
FAU - Rothkotter, Hermann-Josef
AU  - Rothkotter HJ
FAU - Smidt, Hauke
AU  - Smidt H
LA  - eng
SI  - GENBANK/EF378630
SI  - GENBANK/EF378631
SI  - GENBANK/EF378632
SI  - GENBANK/EF378633
SI  - GENBANK/EF378634
SI  - GENBANK/EF378635
SI  - GENBANK/EF378636
SI  - GENBANK/EF378637
PT  - Journal Article
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 804826J2HU (Amoxicillin)
SB  - IM
MH  - Amoxicillin/administration & dosage/*pharmacology
MH  - Animal Feed
MH  - Animals
MH  - Animals, Suckling
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteria/*classification/*drug effects/genetics/growth & development
MH  - DNA, Bacterial/analysis
MH  - *Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Injections, Intramuscular
MH  - Intestines/drug effects/*microbiology
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Swine
EDAT- 2007/11/29 09:00
MHDA- 2007/12/19 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - ismej200729 [pii]
AID - 10.1038/ismej.2007.29 [doi]
PST - ppublish
SO  - ISME J. 2007 Jun;1(2):180-3. doi: 10.1038/ismej.2007.29.