PMID- 18043635
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20151013
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 1
IP  - 3
DP  - 2007 Jul
TI  - Characterization of filamentous bacteria, belonging to candidate phylum KSB3, 
      that are associated with bulking in methanogenic granular sludges.
PG  - 246-55
AB  - A fatal bulking phenomenon was found to occur occasionally in the methanogenic 
      granular sludge of a mesophilic (35-40 degrees C), full-scale upflow anaerobic 
      sludge blanket (UASB) reactor treating organic wastewater discharged from a sugar 
      manufacturing factory. A vast number of filamentous cells were observed in the 
      bulking sludge that were morphologically distinct from the previously recognized 
      anaerobic bulking agent Anaerolinea thermophila. 16S rRNA gene-based analyses of 
      the microbial populations in the bulking sludge revealed that the dominant 
      filamentous organisms were members of proposed candidate bacterial phylum, KSB3. 
      Fluorescence in situ hybridization (FISH) analysis of the healthy sludge granules 
      showed that the KSB3 filaments were the dominant granule surface population 
      suggesting that they are fundamental constituents of the sludge granules and that 
      they occasionally overgrow in the reactor, possibly triggering the filamentous 
      bulking. We surveyed 10 additional mesophilic and thermophilic anaerobic sludges 
      for the presence and diversity of KSB3 populations. Bacteria closely related to 
      the characterized KSB3 filaments were present in two types of mesophilically 
      grown UASB sludge granules treating actual wastewater discharged from 
      sugar-processing industries.
FAU - Yamada, Takeshi
AU  - Yamada T
AD  - Institute for Biological Resources and Functions, National Institute of Advanced 
      Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
FAU - Yamauchi, Toshihiro
AU  - Yamauchi T
FAU - Shiraishi, Koji
AU  - Shiraishi K
FAU - Hugenholtz, Philip
AU  - Hugenholtz P
FAU - Ohashi, Akiyoshi
AU  - Ohashi A
FAU - Harada, Hideki
AU  - Harada H
FAU - Kamagata, Yoichi
AU  - Kamagata Y
FAU - Nakamura, Kazunori
AU  - Nakamura K
FAU - Sekiguchi, Yuji
AU  - Sekiguchi Y
LA  - eng
SI  - GENBANK/AB218870
SI  - GENBANK/AB218871
SI  - GENBANK/AB218872
SI  - GENBANK/AB218873
SI  - GENBANK/AB218874
SI  - GENBANK/AB218875
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070531
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Industrial Waste)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*classification/*genetics/isolation & purification
MH  - Bioreactors/microbiology
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genes, rRNA
MH  - In Situ Hybridization, Fluorescence
MH  - Industrial Waste
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/methods
MH  - Water Purification/methods
EDAT- 2007/11/29 09:00
MHDA- 2008/01/11 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - ismej200728 [pii]
AID - 10.1038/ismej.2007.28 [doi]
PST - ppublish
SO  - ISME J. 2007 Jul;1(3):246-55. doi: 10.1038/ismej.2007.28. Epub 2007 May 31.