PMID- 18043672
OWN - NLM
STAT- MEDLINE
DCOM- 20080114
LR  - 20151013
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 1
IP  - 7
DP  - 2007 Nov
TI  - The diversity of the bacterial communities associated with the azooxanthellate 
      hexacoral Cirrhipathes lutkeni.
PG  - 654-9
AB  - This study examined the symbiotic microbiota of the hexacoral Cirrhipathes 
      lutkeni using traditional plate culture, fluorescence in situ hybridization 
      (FISH) and 16S rDNA characterization. FISH counts for the whole coral (holobiont) 
      showed a major presence of gamma-Proteobacteria (22%) and Actinobacteria (19%), 
      followed by alpha-Proteobacteria (14%), Firmicutes (9%), Cytophaga-Flavobacterium 
      (7%), beta-Proteobacteria (6%) and Chloroflexi (2%). In contrast to the diversity 
      observed by FISH, plate cultures were found to be selective for 
      gamma-Proteobacteria (22 cultures) with the exception of an Actinobacterium. The 
      methods employed in this study detected 76% of all microbes estimated by DAPI 
      staining of C. lutkeni homogenates. The absence of zooxanthellae in this 
      particular hexacoral was confirmed by PCR and spectrophotometry using fresh 
      tissue isolated from the holobiont. This is the first study describing the 
      microbial associations of shallow-water hexacorallia, which opens further insight 
      into coral microbial ecology and may enhance the search for novel natural 
      products in the near future.
FAU - Santiago-Vazquez, Lory Z
AU  - Santiago-Vazquez LZ
AD  - Department of Chemistry and Biochemistry, Center of Excellence in Biomedical and 
      Marine Biotechnology, Florida Atlantic University, Boca Raton, FL, USA.
FAU - Bruck, Thomas B
AU  - Bruck TB
FAU - Bruck, Wolfram M
AU  - Bruck WM
FAU - Duque-Alarcon, Angela P
AU  - Duque-Alarcon AP
FAU - McCarthy, Peter J
AU  - McCarthy PJ
FAU - Kerr, Russell G
AU  - Kerr RG
LA  - eng
SI  - GENBANK/DQ857736
SI  - GENBANK/DQ857737
SI  - GENBANK/DQ857738
SI  - GENBANK/DQ857739
SI  - GENBANK/DQ857740
SI  - GENBANK/DQ857741
SI  - GENBANK/DQ857742
SI  - GENBANK/DQ857743
SI  - GENBANK/DQ857744
SI  - GENBANK/DQ857745
SI  - GENBANK/DQ857746
SI  - GENBANK/DQ857747
SI  - GENBANK/DQ857748
SI  - GENBANK/DQ857749
SI  - GENBANK/DQ857750
SI  - GENBANK/DQ857751
SI  - GENBANK/DQ857752
SI  - GENBANK/DQ857753
SI  - GENBANK/DQ857754
SI  - GENBANK/DQ857755
SI  - GENBANK/DQ857756
SI  - GENBANK/DQ857757
SI  - GENBANK/DQ857758
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20071018
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Actinobacteria/classification/genetics
MH  - Alphaproteobacteria/classification/genetics
MH  - Animals
MH  - Anthozoa/*microbiology
MH  - Bacteria/classification/*genetics/growth & development
MH  - *Biodiversity
MH  - Chloroflexi/classification/genetics
MH  - Cytophagaceae/classification/genetics
MH  - Flavobacterium/classification/genetics
MH  - Gammaproteobacteria/classification/genetics
MH  - Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
EDAT- 2007/11/29 09:00
MHDA- 2008/01/15 09:00
CRDT- 2007/11/29 09:00
PHST- 2007/11/29 09:00 [pubmed]
PHST- 2008/01/15 09:00 [medline]
PHST- 2007/11/29 09:00 [entrez]
AID - ismej200777 [pii]
AID - 10.1038/ismej.2007.77 [doi]
PST - ppublish
SO  - ISME J. 2007 Nov;1(7):654-9. doi: 10.1038/ismej.2007.77. Epub 2007 Oct 18.