PMID- 18046732
OWN - NLM
STAT- MEDLINE
DCOM- 20080314
LR  - 20071206
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 56
IP  - 2
DP  - 2008 Jan 15
TI  - Macrophage attenuation of neuronal excitability: implications for pathogenesis of 
      neurodegenerative disorders.
PG  - 241-6
AB  - Brain macrophages (and microglia) play a crucial role in central nervous system 
      immune and inflammatory responses. They are also critical cells in the 
      pathogenesis of neurodegenerative disorders. To understand how macrophages cause 
      neural cell dysfunction, we investigated the effects of mouse bone marrow-derived 
      macrophages (BMDMs) on rat cortical neuronal physiology in a BMDM-neuronal 
      co-culture system using whole-cell patch clamp techniques. When co-cultured with 
      neuronal cells, BMDMs hyperpolarized the neuronal membrane and attenuated both 
      spontaneous and electrically evoked firings through a decrease in membrane input 
      resistance. The average duration of evoked action potentials (APs) and the 
      latency to fire the APs, in response to a constant depolarizing current 
      injection, were significantly increased by BMDMs. These results indicate that 
      BMDMs attenuate neuronal excitability. Further investigation revealed that BMDMs 
      hyperpolarize neuronal membranes by enhancing neuronal delayed rectifier 
      potassium current (IK), which was blocked by tetraethylammonium. This 
      BMDM-induced attenuation on neuronal excitability may contribute to the 
      pathogenesis of neuronal dysfunction and damage as seen in neurodegenerative 
      disorders.
CI  - Copyright (c) 2007 Wiley-Liss, Inc.
FAU - Wang, Wenwei
AU  - Wang W
AD  - Neurophysiology Laboratory, The Center for Neurovirology and Neurodegenerative 
      Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, 
      USA.
FAU - Hu, Dehui
AU  - Hu D
FAU - Xiong, Huangui
AU  - Xiong H
LA  - eng
GR  - 2 R01 NS041862/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/physiology
MH  - Brain/cytology
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - Dose-Response Relationship, Radiation
MH  - Electric Stimulation/methods
MH  - Embryo, Mammalian
MH  - Macrophages/chemistry/*physiology
MH  - Male
MH  - Membrane Potentials/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/*physiology
MH  - Patch-Clamp Techniques/methods
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2007/11/30 09:00
MHDA- 2008/03/15 09:00
CRDT- 2007/11/30 09:00
PHST- 2007/11/30 09:00 [pubmed]
PHST- 2008/03/15 09:00 [medline]
PHST- 2007/11/30 09:00 [entrez]
AID - 10.1002/glia.20609 [doi]
PST - ppublish
SO  - Glia. 2008 Jan 15;56(2):241-6. doi: 10.1002/glia.20609.