PMID- 18047627 OWN - NLM STAT- MEDLINE DCOM- 20080124 LR - 20131121 IS - 1440-1681 (Electronic) IS - 0305-1870 (Linking) VI - 35 IP - 1 DP - 2008 Jan TI - Methylene blue increases contralateral testicular ischaemia-reperfusion injury after unilateral testicular torsion. PG - 50-4 AB - 1. Testicular ischaemia-reperfusion injury is commonly seen in childhood. Infertility occurs in 25% of patients after unilateral testicular ischaemia. It is has been reported that methylene blue has a positive effect in the reparation of ischaemia-reperfusion injury in different tissues. Therefore, we hypothesized that methylene blue may prevent the hazardous effects of ischaemia-reperfusion injury in testicular tissue after unilateral testicular torsion. 2. Thirty-two prepubertal Wistar-albino rats were divided into four groups. Testicular torsion was created by rotating the right testis 720 degrees in a clockwise direction for 5 h in all groups except for Group C, which was the sham control group. In Group T, bilateral orchiectomy was performed following the torsion period. In Group TD, both testes were removed 5 days after the torsion period. In Group MB, methylene blue (1 mg/kg, i.p.) was administered 40 min before detorsion and once daily over 5 days; then, both testes were harvested. Tissue levels of malondialdehyde (MDA), serum levels of creatine kinase (CK), mean testicular biopsy score (MTBS) and mean seminifer tubule diameter (MSTD) were determined. 3. There was a significant difference in MTBS between Groups T and TD (P < 0.05) in both ipsilateral and contralateral testes. In the contralateral testis, treatment with methylene blue decreased MTBS and MSTD (P < 0.05) and increased MDA levels (P < 0.05). In Group T, mean serum CK concentrations were higher than in any of the other groups (P < 0.05). 4. After 5 h of unilateral testicular torsion and a 5 day reperfusion period, serious tissue damage occurred on both the ipsilateral and contralateral sides. Serum CK concentrations may be an indicator for ischaemia, but not for ischaemia-reperfusion injury. Contrary to our hypothesis, methylene blue increased contralateral testicular damage after unilateral testicular torsion and exacerbated oxidative events. FAU - Inan, Mustafa AU - Inan M AD - Department of Pediatric Surgery, Trakya University Faculty of Medicine, Edirne, Turkey. mustafainan@trakya.edu.tr FAU - Basaran, Umit N AU - Basaran UN FAU - Dokmeci, Dikmen AU - Dokmeci D FAU - Yalcin, Omer AU - Yalcin O FAU - Aydogdu, Nurettin AU - Aydogdu N FAU - Turan, Nesrin AU - Turan N LA - eng PT - Journal Article PL - Australia TA - Clin Exp Pharmacol Physiol JT - Clinical and experimental pharmacology & physiology JID - 0425076 RN - 0 (Enzyme Inhibitors) RN - 4Y8F71G49Q (Malondialdehyde) RN - EC 1.14.13.39 (Nitric Oxide Synthase) RN - EC 1.17.3.2 (Xanthine Oxidase) RN - EC 2.7.3.2 (Creatine Kinase) RN - T42P99266K (Methylene Blue) SB - IM MH - Animals MH - Creatine Kinase/blood MH - Disease Models, Animal MH - Enzyme Inhibitors/*adverse effects MH - Male MH - Malondialdehyde/metabolism MH - Methylene Blue/*adverse effects MH - Nitric Oxide Synthase/antagonists & inhibitors/metabolism MH - Oxidative Stress/drug effects MH - Rats MH - Rats, Wistar MH - Reperfusion Injury/chemically induced/*etiology/metabolism/pathology MH - Spermatic Cord Torsion/*complications/metabolism/pathology MH - Testicular Diseases/chemically induced/*etiology/metabolism/pathology MH - Testis/*drug effects/enzymology/metabolism/pathology MH - Time Factors MH - Xanthine Oxidase/antagonists & inhibitors/metabolism EDAT- 2007/12/01 09:00 MHDA- 2008/01/25 09:00 CRDT- 2007/12/01 09:00 PHST- 2007/12/01 09:00 [pubmed] PHST- 2008/01/25 09:00 [medline] PHST- 2007/12/01 09:00 [entrez] AID - CEP4736 [pii] AID - 10.1111/j.1440-1681.2007.04736.x [doi] PST - ppublish SO - Clin Exp Pharmacol Physiol. 2008 Jan;35(1):50-4. doi: 10.1111/j.1440-1681.2007.04736.x.