PMID- 18047927
OWN - NLM
STAT- MEDLINE
DCOM- 20080225
LR  - 20171116
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 18
IP  - 3
DP  - 2008 Jan
TI  - Activated cells in urine and monocyte chemotactic peptide-1 (MCP-1)--sensitive 
      rejection markers in renal graft recipients.
PG  - 203-7
AB  - Chemokines induced during an acute immune alloresponse cause cellular 
      infiltration of the allograft. These chemokines and cells are excreted with 
      urine. The aim of the study was to assess the diagnostic utility of urinary 
      excretion of monocyte chemotactic peptide-1 and certain cells involved in 
      infiltration i.e. CD3+, CD64+ and HLA-DR+ cells. The study entailed 35 patients 
      with acute renal rejection and 65 with a stable graft function. Urinary sediments 
      were prepared by means of cytospin and stained with anti-CD3, anti-CD64, 
      anti-HLA-DR labeled monoclonal antibodies. Urinary expression of MCP-1 was 
      assayed by ELISA. In the patients with acute rejection MCP-1 level was ten-fold 
      higher than in the patients with a stable graft function (6.1+/-3.4 vs 0.6+/-0.4 
      ng/mg creatinine). The number of CD3+ cells was over 5 times higher than in the 
      non-rejection patients (13.4+/-4.6 vs 2.5+/-2.2). The number of HLA-DR+ cells was 
      6 times higher in the acute rejection patients (15.7+/-5.9 vs 2.5+/-2.7). The 
      number of CD64+ cells was significantly increased in the patients during an acute 
      rejection episode (p<0.0001). CD3+, HLA-DR+ and CD64+ cell counts strongly 
      correlated with urine excretion of MCP-1. The counts of CD3+ and HLA-DR+ cells 
      correlated with Banff score. The assessment of MCP-1 as well as CD3+, CD64+ and 
      HLA-DR+ cells can provide a useful non-invasive device for the diagnosis of acute 
      rejection. A sole assay of HLA-DR+ cell excretion provides enough specificity and 
      sensitivity for the routine monitoring of patients after kidney transplantation, 
      saving costs and time.
FAU - Dubinski, B
AU  - Dubinski B
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, ul. Traugutta 57/59, 50-417 Wroclaw, Poland. 
      bogdanbankowy@poczta.onet.pl
FAU - Boratynska, M
AU  - Boratynska M
FAU - Kopec, W
AU  - Kopec W
FAU - Szyber, P
AU  - Szyber P
FAU - Patrzalek, D
AU  - Patrzalek D
FAU - Klinger, M
AU  - Klinger M
LA  - eng
PT  - Journal Article
DEP - 20070924
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Receptors, IgG)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/urine
MH  - CD3 Complex/analysis
MH  - Chemokine CCL2/*urine
MH  - Creatinine/urine
MH  - Female
MH  - Graft Rejection/*diagnosis/*urine
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Receptors, IgG/immunology/metabolism
MH  - Urine/*cytology
EDAT- 2007/12/01 09:00
MHDA- 2008/02/26 09:00
CRDT- 2007/12/01 09:00
PHST- 2007/01/17 00:00 [received]
PHST- 2007/07/14 00:00 [revised]
PHST- 2007/07/31 00:00 [accepted]
PHST- 2007/12/01 09:00 [pubmed]
PHST- 2008/02/26 09:00 [medline]
PHST- 2007/12/01 09:00 [entrez]
AID - S0966-3274(07)00146-3 [pii]
AID - 10.1016/j.trim.2007.07.005 [doi]
PST - ppublish
SO  - Transpl Immunol. 2008 Jan;18(3):203-7. doi: 10.1016/j.trim.2007.07.005. Epub 2007 
      Sep 24.