PMID- 18049032
OWN - NLM
STAT- MEDLINE
DCOM- 20080407
LR  - 20171116
IS  - 0916-9636 (Print)
IS  - 0916-9636 (Linking)
VI  - 30
IP  - 10
DP  - 2007 Oct
TI  - Albuminuria, expression of nicotinamide adenine dinucleotide phosphate oxidase 
      and monocyte chemoattractant protein-1 in the renal tubules of hypertensive Dahl 
      salt-sensitive rats.
PG  - 991-8
AB  - In chronic renal diseases, experimental and human data suggest that excess 
      albumin filtered through the glomerular capillary barrier is over-reabsorbed by 
      proximal tubular cells, thereby activating these cells and upregulating the 
      expression of chemokines. On the other hand, a high-salt diet has been shown to 
      induce proteinuria in hypertensive Dahl salt-sensitive (DSS) rats, accompanied 
      with the expression of reduced nicotinamide adenine dinucleotide phosphate 
      (NADPH) oxidase in the kidney. In the current study, we therefore examined 
      albuminuria and the expressions of NADPH oxidase and monocyte chemoattractant 
      protein-1 (MCP-1) in the renal tubular cells in hypertensive DSS rats, as well as 
      the effects of the antioxidant N-acetylcysteine (NAC) on each of these 
      parameters. DSS rats were fed a normal-salt diet (0.24% NaCl), a high-salt diet 
      (8% NaCl), or a high-salt diet plus NAC supplementation (15 mg/mL drinking water) 
      for 4 weeks. The high-salt diet provoked an increase in glomerular injuries 
      accompanied with albuminuria and in urinary H2O2 and MCP-1 excretion. 
      Immunohistochemical analysis showed the prominent expression of MCP-1 in the 
      dilated tubular cells, where the NADPH oxidase subunit p47phox was also 
      expressed. The current results suggest that albuminuria caused expression of 
      NADPH oxidase and MCP-1 in the dilated renal tubules, resulting in interstitial 
      inflammation and migration of mononuclear cells in DSS rats, because blockade of 
      albuminuria by NAC counteracted the p47phox and MCP-1 expression.
FAU - Fujii, Shigemoto
AU  - Fujii S
AD  - Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, 
      Japan.
FAU - Zhang, Ling
AU  - Zhang L
FAU - Kosaka, Hiroaki
AU  - Kosaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hypertens Res
JT  - Hypertension research : official journal of the Japanese Society of Hypertension
JID - 9307690
RN  - 0 (Chemokine CCL2)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Albuminuria/*metabolism
MH  - Animals
MH  - Chemokine CCL2/*metabolism
MH  - Gene Expression
MH  - Hypertension/*metabolism
MH  - Immunohistochemistry
MH  - Kidney Tubules/*metabolism
MH  - Male
MH  - NADPH Oxidases/*metabolism
MH  - Rats
MH  - Rats, Inbred Dahl
EDAT- 2007/12/01 09:00
MHDA- 2008/04/09 09:00
CRDT- 2007/12/01 09:00
PHST- 2007/12/01 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2007/12/01 09:00 [entrez]
AID - JST.JSTAGE/hypres/30.991 [pii]
AID - 10.1291/hypres.30.991 [doi]
PST - ppublish
SO  - Hypertens Res. 2007 Oct;30(10):991-8. doi: 10.1291/hypres.30.991.