PMID- 18049813
OWN - NLM
STAT- MEDLINE
DCOM- 20080331
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 390
IP  - 4
DP  - 2008 Feb
TI  - The study of surface properties of an IgE-sensitive aptasensor using an acoustic 
      method.
PG  - 1087-91
AB  - We applied the acoustic transverse shear mode (TSM) method for study of the 
      surface properties of a DNA aptasensor that specifically binds human 
      immunoglobulin E (IgE). The biotinylated 45-mer DNA aptamers were immobilized on 
      the surface of a self-assembled layer composed of a mixture of polyamidoamine 
      dendrimers of the fourth generation with 1-hexadecanetiol covered by neutravidin. 
      Using the TSM method, we studied the kinetics of changes of the series resonant 
      frequency, f(s), and the motional resistance, R(m), of a quartz crystal 
      transducer, used as a support for formation of the sensing layer. We have shown 
      that attachment of the biotinylated DNA aptamers onto the surface covered by 
      neutravidin results in a decrease of f(s), but in an increase of R(m). Similar 
      changes of f(s) and R(m) were observed following addition of IgE. This suggests 
      the contribution of friction forces to the crystal oscillation, which was taken 
      into account in the calculation of the mass changes at the sensor surface 
      following binding processes.
FAU - Snejdarkova, M
AU  - Snejdarkova M
AD  - Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 
      Moyzesova 61, 900 28, Ivanka pri Dunaji, Slovak Republic.
FAU - Svobodova, L
AU  - Svobodova L
FAU - Polohova, V
AU  - Polohova V
FAU - Hianik, T
AU  - Hianik T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071201
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (DNA Primers)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Acoustics
MH  - Aptamers, Nucleotide/*chemistry
MH  - Base Sequence
MH  - *Biosensing Techniques
MH  - DNA Primers
MH  - Immunoglobulin E/*analysis
MH  - Kinetics
MH  - Sensitivity and Specificity
MH  - Surface Properties
EDAT- 2007/12/01 09:00
MHDA- 2008/04/01 09:00
CRDT- 2007/12/01 09:00
PHST- 2007/09/17 00:00 [received]
PHST- 2007/11/09 00:00 [accepted]
PHST- 2007/11/07 00:00 [revised]
PHST- 2007/12/01 09:00 [pubmed]
PHST- 2008/04/01 09:00 [medline]
PHST- 2007/12/01 09:00 [entrez]
AID - 10.1007/s00216-007-1749-2 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2008 Feb;390(4):1087-91. doi: 10.1007/s00216-007-1749-2. Epub 
      2007 Dec 1.