PMID- 18051854
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20191110
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 23
IP  - 5
DP  - 2007 Sep
TI  - [Selection and inhibitory effect analysis of siRNAs specific to ORF2-4 of porcine 
      reproductive and respiratory syndrome virus].
PG  - 794-800
AB  - RNA interference (RNAi) is a powerful tool in gene function research. In order to 
      investigate the role of GP2, GP3 and GP4 of porcine reproductive and respiratory 
      syndrome virus (PRRSV) in the viral replication, small interference RNAs (siRNAs) 
      directed to ORF2, ORF3 and ORF4 were designed and 12 short hairpin RNA (shRNA) 
      expression vectors were constructed (designed as 21,22,23,24,31,32,33,34,41,42,43 
      and 44). Cells treated with shRNA expression vectors were infected by PRRSV. The 
      effective shRNA expression vectors were selected by fluorescent quantatitive PCR 
      (FQ-PCR). The virus titer of supernatant of the cells treated with effective 
      shRNA expression vectors (23,24,31,34 and 41) were reduced by 184 to 4.65 folds 
      compared with that of controls.
FAU - He, Yun-Xia
AU  - He YX
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Hua, Rong-Hong
AU  - Hua RH
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - An, Tong-Qing
AU  - An TQ
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
FAU - Wang, Yun-Feng
AU  - Wang YF
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Genetic Vectors
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA Interference
MH  - RNA, Small Interfering/*genetics/metabolism
MH  - RNA, Viral/chemistry/*metabolism
MH  - Transduction, Genetic
MH  - Viral Proteins/*genetics
MH  - Virus Replication/*genetics
EDAT- 2007/12/07 09:00
MHDA- 2009/09/09 06:00
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
AID - 10.1016/s1872-2075(07)60050-8 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2007 Sep;23(5):794-800. doi: 
      10.1016/s1872-2075(07)60050-8.