PMID- 18052978
OWN - NLM
STAT- MEDLINE
DCOM- 20080306
LR  - 20220818
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 26
IP  - 12
DP  - 2007 Dec
TI  - Olfactory bulb hypoplasia in Prokr2 null mice stems from defective neuronal 
      progenitor migration and differentiation.
PG  - 3339-44
AB  - New neurons are added on a daily basis to the olfactory bulb (OB) of a mammal, 
      and this phenomenon exists throughout its lifetime. These new cells are born in 
      the subventricular zone and migrate to the OB via the rostral migratory stream 
      (RMS). To examine the role of the prokineticin receptor 2 (Prokr2) in 
      neurogenesis, we created a Prokr2 null mouse, and report a decrease in the volume 
      of its OB and also a decrease in the number of bromodeoxyuridine (BrdU)-positive 
      cells. There is disrupted architecture of the OB, with the glomerular layer 
      containing terminal dUTP nick-end labeling (TUNEL) -positive nuclei and also a 
      decrease in tyrosine hydroxylase-positive neurons in this layer. In addition, 
      there are increased numbers of doublecortin-positive neuroblasts in the RMS and 
      increased PSA-NCAM (polysialylated form of the neural cell adhesion molecule) 
      -positive neuronal progenitors around the olfactory ventricle, indicating their 
      detachment from homotypic chains is compromised. Finally, in support of this, 
      Prokr2-deficient cells expanded in vitro as neurospheres are incapable of 
      migrating towards a source of recombinant human prokineticin 2 (PROK2). Together, 
      these findings suggest an important role for Prokr2 in OB neurogenesis.
FAU - Prosser, Haydn M
AU  - Prosser HM
AD  - The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, 
      Cambridge CB10 1SA, UK.
FAU - Bradley, Allan
AU  - Bradley A
FAU - Caldwell, Maeve A
AU  - Caldwell MA
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071204
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Gastrointestinal Hormones)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Neuropeptides)
RN  - 0 (PROK2 protein, human)
RN  - 0 (Prokr2 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialyl neural cell adhesion molecule)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Bromodeoxyuridine
MH  - Cell Count
MH  - *Cell Differentiation
MH  - *Cell Movement/drug effects
MH  - Doublecortin Domain Proteins
MH  - Female
MH  - Gastrointestinal Hormones/pharmacology
MH  - Homozygote
MH  - Humans
MH  - Lactation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neural Cell Adhesion Molecule L1/metabolism
MH  - Neurons/metabolism/*pathology
MH  - Neuropeptides/metabolism/pharmacology
MH  - Olfactory Bulb/*abnormalities/metabolism/*pathology/physiopathology
MH  - Receptors, G-Protein-Coupled/*deficiency/genetics
MH  - Receptors, Peptide/*deficiency/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Sialic Acids/metabolism
MH  - Spheroids, Cellular
MH  - Stem Cells/metabolism/*pathology
MH  - Tyrosine 3-Monooxygenase/metabolism
PMC - PMC2228368
EDAT- 2007/12/07 09:00
MHDA- 2008/03/07 09:00
PMCR- 2008/02/05
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/03/07 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
PHST- 2008/02/05 00:00 [pmc-release]
AID - EJN5958 [pii]
AID - 10.1111/j.1460-9568.2007.05958.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Dec;26(12):3339-44. doi: 10.1111/j.1460-9568.2007.05958.x. 
      Epub 2007 Dec 4.