PMID- 18052982
OWN - NLM
STAT- MEDLINE
DCOM- 20080306
LR  - 20171116
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 26
IP  - 12
DP  - 2007 Dec
TI  - Target regulation of V2R expression and functional maturation in vomeronasal 
      sensory neurons in vitro.
PG  - 3382-94
AB  - Vomeronasal receptors from the V1R and V2R gene families mediate the detection of 
      chemical stimuli such as pheromones via the vomeronasal organ (VNO). The 
      differential expression of vomeronasal receptors might contribute in part to a 
      variety of pheromonal effects, which are different sexually, developmentally and 
      even individually. However, little is known about the mechanisms controlling 
      vomeronasal receptor expression. Cultured vomeronasal sensory neurons (VSNs) bear 
      phenotypic resemblance to the intact VNO but they remain immature. Because 
      indices of VSN maturation are increased by coculture with the target cells for 
      VSNs, accessory olfactory bulb (AOB) neurons, AOB neurons may regulate 
      vomeronasal receptor expression and functional maturation in VSNs. To test this 
      hypothesis, we examined the expression of V2R-type vomeronasal receptors (VR1 and 
      VR4) and chemosensory responsiveness in VNOs cocultured with AOB neurons. 
      Immunoblot and immunocytochemical analysis revealed that the coculture of VNOs 
      with AOB neurons resulted in a greater expression of VR1 and VR4 after 10 days 
      than VNOs cultured alone. Moreover, calcium imaging analysis showed that 
      cocultured VNOs responded to urine components applied iontophoretically into 
      their cavities with a time course similar to the V2R expression, in contrast to 
      singly cultured VNOs that displayed no response. These results demonstrate that 
      AOB neurons induce the expression of vomeronasal receptors in VSNs, allowing them 
      to function.
FAU - Muramoto, Kazuyo
AU  - Muramoto K
AD  - Department of Physiology, Kochi Medical School, Kohasu, Nankoku, Kochi 783-8505, 
      Japan. tkazuyo@kochi-u.ac.jp
FAU - Hashimoto, Mitsuhiro
AU  - Hashimoto M
FAU - Kaba, Hideto
AU  - Kaba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071204
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Pheromone)
RN  - 0 (Receptors, Vasopressin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cellular Senescence/*physiology
MH  - Coculture Techniques
MH  - Embryo, Mammalian
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Neurons, Afferent/metabolism/*physiology
MH  - Olfactory Bulb/cytology
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Pheromone/metabolism
MH  - Receptors, Vasopressin/*metabolism
MH  - Time Factors
MH  - Tissue Distribution
MH  - Urine/chemistry
MH  - Vomeronasal Organ/*cytology/metabolism
EDAT- 2007/12/07 09:00
MHDA- 2008/03/07 09:00
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/03/07 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
AID - EJN5954 [pii]
AID - 10.1111/j.1460-9568.2007.05954.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Dec;26(12):3382-94. doi: 10.1111/j.1460-9568.2007.05954.x. 
      Epub 2007 Dec 4.