PMID- 18055243
OWN - NLM
STAT- MEDLINE
DCOM- 20080403
LR  - 20191210
IS  - 1096-4959 (Print)
IS  - 1096-4959 (Linking)
VI  - 149
IP  - 2
DP  - 2008 Feb
TI  - Antibacterial action of L-amino acid oxidase from the skin mucus of rockfish 
      Sebastes schlegelii.
PG  - 394-400
AB  - L-amino acid oxidase (LAO) shows broadly antibacterial activity against 
      Gram-positive and Gram-negative bacteria by H(2)O(2) generated in the oxidative 
      process of L-amino acids. However, LAO (termed SSAP) isolated from the rockfish 
      Sebastes schlegelii skin mucus acted selectively on Gram-negative bacteria. 
      Therefore, this study was undertaken to clarify the antibacterial action of SSAP 
      as compared with H(2)O(2). SSAP inhibited potently the growth of Aeromonas 
      salmonicida, Photobacterium damselae subsp. piscicida and Vibrio parahaemolyticus 
      with a minimum inhibitory concentration (MIC) of 0.078, 0.16 and 0.63 microg/mL, 
      respectively. H(2)O(2) inhibited the growth of both Gram-positive and 
      Gram-negative bacteria with an MIC ranging from 0.31 to 2.5 mM. When SSAP was 
      incubated with P. damselae subsp. piscicida and Escherichia coli, SSAP was 
      demonstrated to bind to P. damselae subsp. piscicida but not to E. coli by 
      Western blotting and LAO activity measurement. These results show that the 
      bacteria binding activity may be involved in the bacterial cell selectivity of 
      SSAP. Electron microscopic observation of A. salmonicida, P. damselae subsp. 
      piscicida and V. parahaemolyticus revealed that the treatments with SSAP and 
      H(2)O(2) induced cell surface damage to A. salmonicida, remarkable elongation of 
      P. damselae subsp. piscicida bodies and pores into V. parahaemolyticus cells.
FAU - Kitani, Yoichiro
AU  - Kitani Y
AD  - Department of Food Science and Technology, Tokyo University of Marine Science and 
      Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
FAU - Kikuchi, Nobuyo
AU  - Kikuchi N
FAU - Zhang, GuoHua
AU  - Zhang G
FAU - Ishizaki, Shoichiro
AU  - Ishizaki S
FAU - Shimakura, Kuniyoshi
AU  - Shimakura K
FAU - Shiomi, Kazuo
AU  - Shiomi K
FAU - Nagashima, Yuji
AU  - Nagashima Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071109
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Anti-Bacterial Agents)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/metabolism/*pharmacology
MH  - Bacteria/drug effects/growth & development
MH  - Fishes, Poisonous/*metabolism
MH  - Hydrogen Peroxide/pharmacology
MH  - L-Amino Acid Oxidase/metabolism/*pharmacology
MH  - Mucus/*enzymology/metabolism
MH  - Protein Binding
MH  - Skin/*metabolism
EDAT- 2007/12/07 09:00
MHDA- 2008/04/04 09:00
CRDT- 2007/12/07 09:00
PHST- 2007/09/10 00:00 [received]
PHST- 2007/10/27 00:00 [revised]
PHST- 2007/10/31 00:00 [accepted]
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/04/04 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
AID - S1096-4959(07)00415-0 [pii]
AID - 10.1016/j.cbpb.2007.10.013 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):394-400. doi: 
      10.1016/j.cbpb.2007.10.013. Epub 2007 Nov 9.