PMID- 18055544
OWN - NLM
STAT- MEDLINE
DCOM- 20080208
LR  - 20211020
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 171
IP  - 6
DP  - 2007 Dec
TI  - The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system 
      affects cell motility in cultured human podocytes.
PG  - 1789-99
AB  - In crescentic glomerulonephritis (GN), monocyte chemoattractant protein-1 (MCP-1) 
      is overexpressed within the glomeruli, and MCP-1 blockade has renoprotective 
      effects. Adult podocytes are in a quiescent state, but acquisition of a 
      migratory/proliferative phenotype has been described in crescentic GN and 
      implicated in crescent formation. The cognate CC chemokine receptor 2 (CCR2), the 
      MCP-1 receptor, is expressed by other cell types besides monocytes and has been 
      implicated in both cell proliferation and migration. We investigated whether 
      MCP-1 binding to CCR2 can induce a migratory/proliferative response in cultured 
      podocytes. MCP-1 binding to CCR2 enhanced podocyte chemotaxis/haptotaxis in a 
      concentration-dependent manner and had a modest effect on cell proliferation. 
      Closure of a wounded podocyte monolayer was delayed by CCR2 blockade, and CCR2 
      was overexpressed at the wound edge, suggesting a role for CCR2 in driving 
      podocyte migration. Immunohistochemical analysis of kidney biopsies from patients 
      with crescentic GN demonstrated CCR2 expression in both podocytes and cellular 
      crescents, confirming the clinical relevance of our in vitro findings. In 
      conclusion, the MCP-1/CCR2 system is functionally active in podocytes and may be 
      implicated in the migratory events triggered by podocyte injury in crescentic GN 
      and other glomerular diseases.
FAU - Burt, Davina
AU  - Burt D
AD  - Diabetic Nephropathy Laboratory, Department of Internal Medicine, University of 
      Turin, Turin, 10126, Italy. davina.burt@unito.it
FAU - Salvidio, Gennaro
AU  - Salvidio G
FAU - Tarabra, Elena
AU  - Tarabra E
FAU - Barutta, Federica
AU  - Barutta F
FAU - Pinach, Silvia
AU  - Pinach S
FAU - Dentelli, Patrizia
AU  - Dentelli P
FAU - Camussi, Giovanni
AU  - Camussi G
FAU - Perin, Paolo Cavallo
AU  - Perin PC
FAU - Gruden, Gabriella
AU  - Gruden G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071130
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CCL2)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, CCR2)
RN  - 0 (nephrin)
SB  - IM
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism/pharmacology
MH  - *Chemotaxis
MH  - Glomerulonephritis/metabolism/*pathology
MH  - Humans
MH  - Kidney/chemistry/metabolism/pathology
MH  - Membrane Proteins/metabolism
MH  - Podocytes/drug effects/metabolism/*pathology
MH  - Receptors, CCR2/analysis/*metabolism
PMC - PMC2111103
EDAT- 2007/12/07 09:00
MHDA- 2008/02/09 09:00
PMCR- 2008/06/01
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/02/09 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - S0002-9440(10)62441-8 [pii]
AID - 10.2353/ajpath.2007.070398 [doi]
PST - ppublish
SO  - Am J Pathol. 2007 Dec;171(6):1789-99. doi: 10.2353/ajpath.2007.070398. Epub 2007 
      Nov 30.