PMID- 18055611
OWN - NLM
STAT- MEDLINE
DCOM- 20080307
LR  - 20240405
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 19
IP  - 11
DP  - 2007 Nov
TI  - Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a 
      high molecular mass complex containing the RNA stabilization factor Nac2 and the 
      translational activator RBP40.
PG  - 3627-39
AB  - Gene expression in chloroplasts is regulated mainly at the posttranscriptional 
      level. In the green alga Chlamydomonas reinhardtii, synthesis of the D2 protein 
      (PsbD), which is the rate-determining subunit for the assembly of photosystem II, 
      depends on the RNA stability factor Nac2. In addition, the RNA binding protein 
      RBP40 has been implicated in translational control via a U-rich element in the 5' 
      untranslated region (5'UTR) of the psbD mRNA. Here, we report the identification 
      of the RBP40 gene based on mass spectrometric analysis of its purified product. 
      Unexpectedly, this was found to be identical to the previously described RNA 
      binding protein RB38, which had been suggested to be involved in the regulation 
      of D1 protein synthesis. However, we show that RBP40 binds to the psbD 5'UTR in a 
      Nac2-dependent fashion both in vitro and in vivo. Molecular characterization of 
      RBP40 RNA interference lines confirmed that RBP40 specifically affects the 
      initiation of D2 synthesis. Native polyacrylamide gel electrophoresis, 
      coimmunoprecipitation, and sedimentation analyses revealed that Nac2 and RBP40 
      form parts of a complex of 550 kD that is displaced from the psbD mRNA prior to 
      polysome assembly. Together, these data indicate that the processes of 
      5'UTR-mediated RNA stabilization and translation initiation are tightly coupled 
      in Chlamydomonas.
FAU - Schwarz, Christian
AU  - Schwarz C
AD  - Molekulare Pflanzenwissenschaften, Ludwig-Maximilians Universitat Munchen, 80638 
      Munich, Germany.
FAU - Elles, Ingolf
AU  - Elles I
FAU - Kortmann, Jens
AU  - Kortmann J
FAU - Piotrowski, Markus
AU  - Piotrowski M
FAU - Nickelsen, Jorg
AU  - Nickelsen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071130
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Algal Proteins)
RN  - 0 (Photosystem II Protein Complex)
RN  - 0 (RNA, Algal)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Algal Proteins/chemistry/isolation & purification/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chlamydomonas reinhardtii/genetics/*metabolism
MH  - Chloroplasts/metabolism
MH  - Immunoprecipitation
MH  - Mass Spectrometry
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Photosystem II Protein Complex/*biosynthesis
MH  - Polyribosomes/metabolism
MH  - Protein Binding
MH  - *Protein Biosynthesis
MH  - RNA Interference
MH  - *RNA Stability
MH  - RNA, Algal/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/*metabolism
PMC - PMC2174876
EDAT- 2007/12/07 09:00
MHDA- 2008/03/08 09:00
PMCR- 2008/11/01
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/03/08 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
PHST- 2008/11/01 00:00 [pmc-release]
AID - tpc.107.051722 [pii]
AID - 051722 [pii]
AID - 10.1105/tpc.107.051722 [doi]
PST - ppublish
SO  - Plant Cell. 2007 Nov;19(11):3627-39. doi: 10.1105/tpc.107.051722. Epub 2007 Nov 
      30.