PMID- 18055810
OWN - NLM
STAT- MEDLINE
DCOM- 20080129
LR  - 20220208
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 48
IP  - 12
DP  - 2007 Dec
TI  - Macrophages are important determinants of acute ocular HSV-1 infection in 
      immunized mice.
PG  - 5605-15
AB  - PURPOSE: To determine the effect of macrophage depletion on herpes simplex virus 
      type (HAV)-1 replication in the eye and on the establishment of latency in 
      trigeminal ganglia (TG) of immunized and ocularly infected mice. METHODS: BALB/c 
      mice were immunized with five HSV-1 glycoprotein DNA genes or were sham 
      immunized. The virulent HSV-1 strain KOS was used as a positive vaccine control. 
      Immunized mice were depleted of their macrophages by dichloromethylene 
      diphosphonate (Cl(2)MDP) injection. After ocular infection with the HSV-1 strain 
      McKrae, virus replication in the eye, blepharitis, corneal scarring, and 
      dermatitis were determined. Finally, the copy numbers of latency-associated 
      transcript (LAT) and CD4(+) and CD8(+) T-cell transcripts in the TGs of surviving 
      mice 30 days after infection were determined by RT-PCR. RESULTS: Depletion of 
      macrophages in immunized mice increased HSV-1 replication in the eye of infected 
      mice between days 1 and 5 after ocular infection. Depletion of macrophages did 
      not alter the HSV-1-induced death or corneal scarring in immunized mice. 
      Macrophage depletion, however, resulted in increased blepharitis in immunized 
      mice. Finally, macrophage depletion had no effect on the establishment of latency 
      in immunized mice, as the TGs from both depleted and mock-depleted mice were 
      negative for the presence of the LAT transcript. CONCLUSIONS: In immunized mice 
      during primary HSV-1 ocular infection, macrophages play an important role in 
      vaccine efficacy against HSV-1 replication in the eye and blepharitis in infected 
      mice. During the latent stage of HSV-1 infection, however, macrophage depletion 
      failed to have any observable effect on HSV-1 latency in the TGs of infected 
      mice.
FAU - Mott, Kevin
AU  - Mott K
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratories, Cedars-Sinai Medical Center Burns and Allen Research Institute, Los 
      Angeles, California 90048, USA.
FAU - Brick, David J
AU  - Brick DJ
FAU - van Rooijen, Nico
AU  - van Rooijen N
FAU - Ghiasi, Homayon
AU  - Ghiasi H
LA  - eng
GR  - R01 EY014966/EY/NEI NIH HHS/United States
GR  - EY14966/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
RN  - 0 (Herpes Simplex Virus Vaccines)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 3OWL53L36A (Mannitol)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Blepharitis/prevention & control/virology
MH  - Cytokines/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Freund's Adjuvant/therapeutic use
MH  - Herpes Simplex Virus Vaccines/*therapeutic use
MH  - Herpesvirus 1, Human/*physiology
MH  - Keratitis, Dendritic/mortality/*prevention & control/virology
MH  - Macrophages/*physiology
MH  - Mannitol/analogs & derivatives/therapeutic use
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Vaccination
MH  - Vaccines, DNA/*therapeutic use
MH  - Viral Envelope Proteins/genetics/immunology
MH  - Virus Latency
MH  - Virus Replication/physiology
EDAT- 2007/12/07 09:00
MHDA- 2008/01/30 09:00
CRDT- 2007/12/07 09:00
PHST- 2007/12/07 09:00 [pubmed]
PHST- 2008/01/30 09:00 [medline]
PHST- 2007/12/07 09:00 [entrez]
AID - 48/12/5605 [pii]
AID - 10.1167/iovs.07-0894 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2007 Dec;48(12):5605-15. doi: 10.1167/iovs.07-0894.