PMID- 18067084
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20071210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 6
DP  - 2007 Dec
TI  - [Fluorescence in situ hybridization identifies complex chromosomal aberrations in 
      multiple myeloma].
PG  - 685-8
AB  - OBJECTIVE: To explore the value of the technique of multiplex fluorescence in sit 
      hybridization (M-FISH) combined with interphase fluorescence in situ 
      hybridization (FISH) in the identification of the chromosomal aberrations in 
      multiple myeloma (MM) and to investigate the frequency of 13q14 deletion, IgH 
      translocations and 17p13 deletion. METHODS: Seven MM patients with complex 
      chromosomal abnormalities (CCAs) were analyzed by combining the technique of 
      conventional cytogenetics (CC) with M-FISH and FISH. RESULTS: M-FISH identified 
      the aberrations which were undetected by CC, including twelve kinds of numeral 
      aberrations and twenty-nine kinds of structural aberrations, In addition, 
      abnormalities of chromosome 1, chromosomes 13 deletion and IgH translocations 
      were the most frequent aberrations. Using the LSI D13S319 probe specific for 
      13q14, we observed a deletion of 13q14 in 6 MM patients; using the LSI p53 probe 
      specific for 17p13, we observed p53 deletion in 4 MM patients; using the LSI 
      IGHC/IGHV probe specific for 14q32, we observed a translocation involving 14q32 
      in 5 MM patients (43.5%), two translocations in two cases (case 6 and 7). 
      CONCLUSION: M-FISH combined with FISH could refine the cytogenetics of MM 
      patients and detect the missed abnormalities or correct the misidentified 
      abnormalities analyzed by CC. It provides an ideal method for the research of 
      chromosomal aberrations in MM.
FAU - Liu, Shu-yan
AU  - Liu SY
AD  - Department of Hematology, the Sir Run Run Shaw Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, 310016 PR China.
FAU - Huang, Jin-wen
AU  - Huang JW
FAU - Zhang, Jin
AU  - Zhang J
FAU - Du, Hua-ping
AU  - Du HP
FAU - Jiang, Hao
AU  - Jiang H
FAU - Li, Jian-yong
AU  - Li JY
FAU - Xue, Yong-quan
AU  - Xue YQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 13
MH  - Female
MH  - Humans
MH  - Immunoglobulin Heavy Chains
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - *Translocation, Genetic
EDAT- 2007/12/11 09:00
MHDA- 2009/04/28 09:00
CRDT- 2007/12/11 09:00
PHST- 2007/12/11 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2007/12/11 09:00 [entrez]
AID - 940624156 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Dec;24(6):685-8.