PMID- 18067090
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20071210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 6
DP  - 2007 Dec
TI  - [Analysis of chromosome mosaicism in preimplantation embryos by using 2 
      sequential rounds of fluorescence in situ hybridization].
PG  - 706-8
AB  - OBJECTIVE: To investigate the mechanism and factors affecting mosaicism in human 
      preimplantation embryos by using 2 sequential rounds of fluorescence in situ 
      hybridization(FISH). METHODS: Totally 51 normal fertilized embryos, which were 
      not suitable for embryo transfer and cryopreservation, were analyzed on day 3 
      after fertilization by using two sequential rounds of FISH. Chromosomes 13, 16, 
      18, 21, 22, X and Y were analyzed. RESULTS: Among 51 embryos, 16 (31.4%) were 
      mosaic, 12 (23.5%) were chaotic, and the remaining were either normal (27.5%) or 
      non-mosaic abnormal (17.6%). The incidence of mosaic embryos was related to 
      embryo developmental stage, for the incidence of mosaicism increased from 12.5% 
      in embryos <or= 4 cell stage to 40.0% in 5-8 cell stage embryos. The aneuploidy 
      rate for the patients over 35 years of age was significantly higher than that of 
      the patients under 35 years (57.1% vs 23.3%). CONCLUSION: Mosaicism is common in 
      human preimplantaion embryos, which may be one of the important factors affecting 
      the success rates in IVF-ET. Most of the chromosomal abnormalities can be 
      identified by two sequential rounds of FISH.
FAU - Ren, Xiu-lian
AU  - Ren XL
AD  - Reproductive Medical Center, the First Affiliated Hospital, SUN Yat-sen 
      University, Guangzhou, Guangdong, 510080 PR China.
FAU - Xu, Yan-wen
AU  - Xu YW
FAU - Zhou, Can-quan
AU  - Zhou CQ
FAU - Zhuang, Guang-lun
AU  - Zhuang GL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - *Aneuploidy
MH  - *Blastocyst
MH  - Chromosomes, Human
MH  - Embryo Transfer
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mosaicism/chemically induced/*embryology
MH  - *Preimplantation Diagnosis
EDAT- 2007/12/11 09:00
MHDA- 2009/04/28 09:00
CRDT- 2007/12/11 09:00
PHST- 2007/12/11 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2007/12/11 09:00 [entrez]
AID - 940624162 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Dec;24(6):706-8.