PMID- 18068810
OWN - NLM
STAT- MEDLINE
DCOM- 20080422
LR  - 20080226
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 122
IP  - 1-2
DP  - 2008 Mar 15
TI  - Lack of detection of circulating skin-specific IgE autoantibodies in dogs with 
      moderate or severe atopic dermatitis.
PG  - 182-7
AB  - Human patients with atopic dermatitis (AD) commonly exhibit IgE reactivity to 
      cutaneous self-antigens. The presence of serum IgE autoantibodies appears to 
      correlate with disease severity, and it is suspected to reflect or contribute to 
      tissue damage. The objective of this study was to determine whether IgE 
      autoantibodies specific for cutaneous antigens could be detected in the serum of 
      dogs with AD. Serum was collected from 19 dogs with untreated moderate to severe 
      AD and four specific-pathogen free (SPF) dogs. Indirect immunofluorescence was 
      performed using normal canine skin collected at four different locations (concave 
      ear, nose, medial thigh and lateral thorax), while Western immunoblotting was 
      done using normal canine ear pinna epidermal and dermal extracts and reducing 
      conditions. In both methods, IgE was detected using a monoclonal antibody 
      specific for heat stable epitopes of canine IgE. At 1:10 dilution, specific IgE 
      autoantibodies against cutaneous autoantigens were not detected, with either 
      method, in AD and SPF canine sera. Either IgE autoreactivity is not associated 
      with moderate to severe AD in dogs, or the methods employed herein were not 
      sensitive enough to permit IgE autoantibody detection.
FAU - Olivry, Thierry
AU  - Olivry T
AD  - North Carolina State University, Raleigh, NC 27606, USA. Thierry-Olivry@ncsu.edu
FAU - Dunston, Stanley M
AU  - Dunston SM
FAU - Pluchino, Kristen
AU  - Pluchino K
FAU - Porter, Kyleigh
AU  - Porter K
FAU - Hammerberg, Bruce
AU  - Hammerberg B
LA  - eng
PT  - Journal Article
DEP - 20071109
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Autoantibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Autoantibodies/*blood
MH  - Dermatitis, Atopic/*veterinary
MH  - Dog Diseases/*immunology
MH  - Dogs
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Immunoblotting
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - Skin/*immunology
EDAT- 2007/12/11 09:00
MHDA- 2008/04/23 09:00
CRDT- 2007/12/11 09:00
PHST- 2007/09/08 00:00 [received]
PHST- 2007/11/01 00:00 [revised]
PHST- 2007/11/01 00:00 [accepted]
PHST- 2007/12/11 09:00 [pubmed]
PHST- 2008/04/23 09:00 [medline]
PHST- 2007/12/11 09:00 [entrez]
AID - S0165-2427(07)00395-9 [pii]
AID - 10.1016/j.vetimm.2007.11.003 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):182-7. doi: 
      10.1016/j.vetimm.2007.11.003. Epub 2007 Nov 9.