PMID- 18069675
OWN - NLM
STAT- MEDLINE
DCOM- 20080515
LR  - 20180913
IS  - 1078-0998 (Print)
IS  - 1078-0998 (Linking)
VI  - 14
IP  - 3
DP  - 2008 Mar
TI  - Autoantibodies against ubiquitination factor E4A (UBE4A) are associated with 
      severity of Crohn's disease.
PG  - 310-7
AB  - BACKGROUND: Identification of a disease- and organ-specific autoantigen can 
      potentially contribute to understanding molecular mechanisms involved in Crohn's 
      disease (CD) and lead to development of clinically useful markers. The aim was to 
      identify potential intestinal autoantigens specific to patients with CD and 
      evaluate their diagnostic value. METHODS: A cDNA expression library from normal 
      terminal ileum was created and screened with pooled sera from 3 randomly selected 
      patients with CD. For evaluation of the diagnostic value of antibody screening, 
      serum samples obtained from 39 patients with CD, 28 patients with ulcerative 
      colitis (UC), and 60 healthy controls were examined for IgG against the strongest 
      clone. RESULTS: We identified an intestinal cDNA clone encoding ubiquitination 
      factor E4A (UBE4A), a U-box-type ubiquitin-protein ligase. The prevalence of 
      anti-UBE4A IgG in patients with CD was significantly higher than that in patients 
      with UC or healthy controls (46.2% versus respectively 7.1%, P = 0.0006; 3.3%, P 
      < 0.0001). Anti-UBE4A-positive patients with CD were more likely to require 
      surgery (P = 0.0013). The level of anti-UBE4A IgG was correlated with disease 
      activity (r = 0.777, P < 0.0001) and associated with stricturing or penetrating 
      disease (P = 0.0028). Immunohistochemical studies showed upregulation of UBE4A in 
      enteroendocrine cells of inflamed ileal mucosa with CD. CONCLUSIONS: Anti-UBE4A 
      antibodies are potentially useful markers for detection and prediction of 
      clinical activity and outcome in patients with CD.
FAU - Sakiyama, Toshio
AU  - Sakiyama T
AD  - Department of Digestive and Life-style Related Diseases, Kagoshima University 
      Graduate School of Medical and Dental Sciences, Kagoshima, Japan. 
      sakiyama@po.synapse.ne.jp
FAU - Fujita, Hiroshi
AU  - Fujita H
FAU - Tsubouchi, Hirohito
AU  - Tsubouchi H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Inflamm Bowel Dis
JT  - Inflammatory bowel diseases
JID - 9508162
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Immunoglobulin G)
RN  - EC 2.3.2.27 (UBE4A protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Adult
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Autoantibodies/*immunology
MH  - Biomarkers/metabolism
MH  - Crohn Disease/*immunology/metabolism/pathology
MH  - DNA, Complementary/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Ileum/immunology/pathology
MH  - Immunoglobulin G/immunology
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/immunology/pathology
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Severity of Illness Index
MH  - Ubiquitin-Protein Ligases/biosynthesis/*genetics/*immunology
EDAT- 2007/12/12 09:00
MHDA- 2008/05/16 09:00
CRDT- 2007/12/12 09:00
PHST- 2007/12/12 09:00 [pubmed]
PHST- 2008/05/16 09:00 [medline]
PHST- 2007/12/12 09:00 [entrez]
AID - 10.1002/ibd.20328 [doi]
PST - ppublish
SO  - Inflamm Bowel Dis. 2008 Mar;14(3):310-7. doi: 10.1002/ibd.20328.