PMID- 18070082
OWN - NLM
STAT- MEDLINE
DCOM- 20080721
LR  - 20191210
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 17
IP  - 6
DP  - 2008 Jun
TI  - A graft model for hair development.
PG  - 512-8
AB  - Follicular cell implantation (FCI) is an experimental cell therapy for the 
      treatment of hair loss that uses cultured hair follicle cells to induce new hair 
      formation. This treatment is based on the demonstration that adult dermal papilla 
      cells (DPC) retain the hair inductive capacity they acquired during hair 
      morphogenesis in the embryo. For FCI, hair inductive cells are isolated from 
      scalp biopsies and then propagated in culture in order to provide enough cells to 
      generate many new follicles from a few donor follicles. Following expansion in 
      culture, the cells are implanted into the scalp where they induce the formation 
      of new follicles. Because the process relies on the ability to retain the 
      potential for hair induction during the expansion of DPC in culture, we sought a 
      consistent, reliable and easily performed in vivo assay in which to test hair 
      induction. In this study, we describe a simple graft model that supports hair 
      morphogenesis. The assay combines dermal cells with embryonic mouse epidermis 
      that provides the keratinocyte component of induced follicles. The grafts are 
      placed under a protective skin flap in the host athymic mouse where the cells 
      will form a skin graft with hair if the dermal cells are hair inductive DPC. 
      Using the assay, freshly isolated and cultured mouse embryo dermal cells as well 
      as cultured dermal papilla cells from other species all induced hair formation. 
      The induced hairs were aesthetically indistinguishable from those of the 
      epidermal donor in length, thickness, and pigmentation, and they were 
      histologically normal.
FAU - Qiao, Jizeng
AU  - Qiao J
AD  - Intercytex Ltd, 175-E New Boston Street, Woburn, MA, USA.
FAU - Philips, Erica
AU  - Philips E
FAU - Teumer, Jeff
AU  - Teumer J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20071207
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Cells, Cultured
MH  - Dermis/cytology/*transplantation
MH  - Epidermis/embryology/*transplantation
MH  - Female
MH  - Fibroblasts/*transplantation
MH  - Hair/*growth & development/transplantation
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Nude
MH  - Models, Biological
MH  - Pregnancy
MH  - Skin Transplantation/*methods
MH  - Surgical Flaps
EDAT- 2007/12/12 09:00
MHDA- 2008/07/22 09:00
CRDT- 2007/12/12 09:00
PHST- 2007/12/12 09:00 [pubmed]
PHST- 2008/07/22 09:00 [medline]
PHST- 2007/12/12 09:00 [entrez]
AID - EXD661 [pii]
AID - 10.1111/j.1600-0625.2007.00661.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2008 Jun;17(6):512-8. doi: 10.1111/j.1600-0625.2007.00661.x. Epub 
      2007 Dec 7.